左归丸对小鼠自身免疫性卵巢损伤的保护作用

作者	单位
朱玲	南方医科大学中医系
罗颂平	广州中医药大学第一临床医学院妇科
许丽绵	广州中医药大学第一临床医学院妇科
朱初良	广西柳州市中医院

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中文摘要:

目的探讨补肾复方左归丸对卵巢早衰(premature ovarian failure,POF)的影响。方法以SD雌性大鼠卵巢组织制备卵巢抗原,皮下多点注射免疫BALB/C雌性小鼠,建立卵巢早衰模型。于造模过程中不同时间点用左归丸处理小鼠,以泼尼松为阳性对照,放射免疫法检测外周血卵泡刺激素(FSH)、雌二醇水平(E2),酶联免疫法检测外周血抗卵巢抗体(AoAb),原位杂交法检测卵巢组织生长分化因子-9(GDF-9)mRNA的表达。结果POF小鼠卵巢间质可见淋巴细胞浸润,外周血FSH升高,E2下降,AoAb阳性,卵母细胞GDF-9mRNA阳性细胞数减少,生长卵泡及成熟卵泡数减少。左归丸能减缓POF小鼠外周血FSH升高,提高体内E2水平,抑制AoAb产生,提高卵母细胞GDF-9 mRNA阳性细胞数,使生长卵泡及成熟卵泡数增加,早期用药比后期用药作用显著。结论左归丸能改善卵巢免疫性炎症的损伤,对免疫性卵巢早衰具有治疗作用。

英文摘要:

ObjectiveTo probe the effect of Zuogui pill （ZGP）, a Chinese compound recipe for tonifying Shen, on ovarian function in mice with premature ovarian failure （POF）. MethodsBALB/C female mice model of POF was established by multiple sites subcutaneous injection of ovarian antigen elicited with ovarian tissue of SD female rats, and treated with ZGP at different time points in the modeling, with prednisone as positive control. The levels of follicle-stimulating hormone （FSH） and estradiol （E₂） in peripheral blood were measured with radioimmunoassay, and ovarian antibody （AoAb） was determined by enzyme linked immunosorbent assay. The mRNA expression of ovarian growth and differentiation factor-9 was detected with in situ hybridization. ResultsPOF model mice manifested such abnormalities as increased FSH, decreased E₂, and positive AoAb in peripheral blood, with lymphocytes infiltration in ovarian mesanchyma, reduction of GDF-9 mRNA positive oocytes, and decrease of growing and mature follicles. ZGP could reduce the increase of FSH, increase the level of E₂, inhibit the production of AoAb, raise the GDF-9 mRNA positive cells of oocytes, increase the number of growing and mature follicles. The clinical efficacy was more significant in early stage than in advanced stage. ConclusionZGP can improve immune inflammatory injury of ovary, and shows therapeutic effect on POF.

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