银杏平颤方及其拆方对帕金森病模型小鼠脑内线粒体酶复合体活性的影响

作者	单位
孙红梅	北京中医药大学基础医学院解剖教研室北京100029
白丽敏	北京中医药大学基础医学院解剖教研室北京100029
张军	北京中医药大学基础医学院解剖教研室北京100029
吴海霞	北京中医药大学基础医学院解剖教研室北京100029
许红	北京中医药大学基础医学院解剖教研室北京100029
崔龙	北京中医药大学基础医学院解剖教研室北京100029

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中文摘要:

目的探讨银杏平颤方防治帕金森病的机理及其阻止帕金森病模型小鼠黑质多巴胺神经元凋亡的途径。方法取雄性C57BL/6J小鼠,分为正常组、模型组和4个中药组(即全方组、解毒方组、平肝方组和化瘀方组)。采用1-甲基-4-苯基-1,2,3,6四氢吡啶腹腔注射建立帕金森病模型,分别于用药14天和28天处死,测定小鼠脑线粒体酶复合体Ⅰ、Ⅱ和Ⅳ的活性。结果与正常组比较,模型组小鼠脑线粒体酶复合体Ⅰ和Ⅳ的活性明显降低(P<0.05,P<0.01),Ⅱ的活性无明显变化;与模型组比较,全方组和化瘀方组14天时脑线粒体酶复合体Ⅰ活性均显著提高(P<0.05),解毒方组14天、平肝方组28天、化瘀方组两个时段复合体Ⅱ的活性均显著升高(P<0.05或P<0.01);与模型组比较,中药各组在14天时复合体Ⅳ活性均升高(P<0.05,P<0.01),平肝方组和化瘀方组在28天时显著升高(P<0.05)。结论银杏平颤方及其拆方可能通过部分抑制脑内线粒体酶复合体活性下降,维持线粒体的功能,阻止中脑多巴胺神经元的凋亡,以减缓帕金森病的进展。

英文摘要:

ObjectiveTo investigate the mechanisms of Yinxing Pingchan recipe (YXPC) and its components, i.e. the components for detoxicating (A), for calming liver (B) and for dissolving blood stasis(C), in preventing and treating Parkinson’s disease, and the path of its inhibition on nigrostriatal dopaminergic neuron (DAn) apoptosis in model mice of Parkinson’s disease. Methods Male C_ 57 BL/6J mice were divided into the normal group, the model group and four Chinese medicinal groups, that is, the YXPC group, and Group A, B and C, treated with YXPC and its components A, B and C respectively. Mouse model of Parkinson’s disease was established by intraperitoneal injection with 1-meth1-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP). All mice were sacrificed in 2 batches at the 14th and the 28th day respectively. The activity of mitochondrial enzyme complexⅠ,Ⅱand Ⅳ (MECⅠ,Ⅱand Ⅳ) in the brain of mice were measured, respectively. Results As compared with the normal group, the activity of MECⅠand Ⅳ in brain was significantly lower (P<0.05 or P<0.01), and that of MECⅡ had no obvious change in the model group. As compared with the model group, the activity of MECⅠwas significantly higher in YXPC group and Group C at the 14th day (P<0.05), while the activity of MECⅡin Group A at the 14th day , Group B at the 28th day and Group C at both 14th and 28th day was significantly lower (P<0.05 or P<0.01). Activity of MEC Ⅳ in the four Chinese medicinal groups at the 14th day all significantly increased (P<0.05 or P<0.01), and retained at high level in Group B and Group C at the 28th day (P<0.05). Conclusion YXPC and its components can maintain the mitochondrial function by partial inhibiting the activity of its enzyme complex, preventing DAn apoptosis to slow down the progress of Parkinson’s disease.

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