| 潘志恒,谢瑶,何宏文,武敬,何义华.大黄虫丸对大鼠肝星状细胞基质金属蛋白酶表达及活性的影响[J].中国中西医结合杂志,2005,(12):1100-1103 |
| 大黄虫丸对大鼠肝星状细胞基质金属蛋白酶表达及活性的影响 |
| Effects of Dahuang Zhechong Pill on Expression and Activity of Matrix Metalloproteinase in Rats’ Hepatic Stellate Cells |
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| DOI： |
| 中文关键词: 大黄虫丸 肝星状细胞 间质胶原酶 明胶酶A |
| 英文关键词:Dahuang Zhechong Pill hepatic stellate cells matrix metalloproteinase-1 matrix metalloproteinase-2 |
| 基金项目:广东省中医药管理局科研基金项目(No.1040053);国家中医药管理局科研基金项目(No.0405JP51) |
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| 中文摘要: |
| 目的观察大黄虫丸对大鼠肝星状细胞间质胶原酶(MMP1)基因表达及分泌到培养基中的明胶酶A(MMP2)活性的影响。方法分离培养大鼠肝星状细胞(HSC),制备大黄虫丸大鼠药物血清,温育HSC。逆转录多聚酶链反应(RTPCR)法观察MMP1的表达。酶谱法检测MMP2的活性。结果大黄虫丸药物血清可明显促进HSC对MMP1的基因表达,同时可明显增加HSC合成的MMP2的含量和活性(P<0.05)。结论大黄虫丸的抗肝纤维化作用与其促进HSC的MMP1基因表达,增加MMP2的含量和活性有关。 |
| 英文摘要: |
| Objective To observe the effects of Dahuang Zhechong Pill (DZP) on the gene expression of matrix metalloproteinase-1 (MMP-1) in rats’ hepatic stellate cells (HSC) and activity of matrix metalloproteinase-2 (MMP-2) that secreted into the culture base. Methods [WT5BZ] HSC were isolated from the liver of normal rats and incubated with DZP-contained drug serum. The expression of MMP-1 in the HSC was detected by quantitative reverse-transcription polymerase chain reaction (RT-PCR). The activity of MMP-2 was examined by zymography. Results DZP-contained drug serum could obviously promote the gene expression of MMP-1 in HSC. In the meantime, it could obviously increase the content and activity of MMP-2 synthesized by HSC (P<0.05). Conclusion The anti-fibrosis action of DZP was correlated to the promotion of HSC’s gene expression of MMP-1 and increasing of the contents and activity of MMP-2. |
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