| 徐意,田金洲,盛树力,时晶,姬志娟,尹军祥,赵志炜.金思维对老年性痴呆模型大鼠突触后致密区蛋白的影响[J].中国中西医结合杂志,2006,(1):54-57 |
| 金思维对老年性痴呆模型大鼠突触后致密区蛋白的影响 |
| Effect of GETO on Expression of Protein in Postsynaptic Dense Zone of Alzheimer’s Disease Model Rats |
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| DOI： |
| 中文关键词: 金思维 Alzheimer病 空间学习记忆 Morris水迷宫 突触后致密区 PSD-95 Shank-1 |
| 英文关键词:GETO Alzheimer disease learning and memory Morris water maze postsynaptic dense zone PSD-95 Shank-1 |
| 基金项目:教育部科学技术研究重大项目(No.10408);国家重点基础研究发展计划(973计划,No.2003CB517104) |
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| 中文摘要: |
| 目的观察Aβ对突触后致密区PSD-95和Shank-1蛋白的改变以及中药金思维对其影响。方法应用凝聚态Aβ1-42在大鼠海马内注射以建立老年性痴呆(Alzheimer’sdisease,AD)动物模型;4周后采用Morris水迷宫测试大鼠学习记忆功能;采用免疫组化法和计算机图像分析技术测定海马CA1区突触后致密区PSD-95和Shank-1蛋白阳性神经元数目及光密度值。结果Morris水迷宫实验:模型组大鼠在定位航行试验中隐匿平台平均逃避潜伏期较正常组显著延长,空间探索试验中跨越原平台位置次数明显减少(P<0·01);金思维组大鼠平均逃避潜伏期较模型组显著缩短,跨越原平台位置次数明显增加(P<0·01);但与多奈哌齐组和正常组比较差异无显著性(P>0·05)。免疫组化实验:模型组和正常组大鼠海马CA1区PSD-95阳性细胞数、Shank-1阳性细胞数比较差异有显著性(P<0·01);金思维组海马CA1区PSD-95、Shank-1阳性细胞数与模型组比较差异有显著性(P<0·05),但与多奈哌齐组和正常组比较差异无显著性(P>0·05)。结论金思维对Alzheimer病模型大鼠的学习记忆功能减退具有改善作用,可能与促进海马神经元PSD-95、Shank-1的表达,恢复突触的结构和功能,增强突触的可塑性有关。 |
| 英文摘要: |
| Objective To observe the effect of GETO on expression of PSD-95 and Shank-1 protein in postsynaptic dense zone in Alzheimer disease (AD) model rats. Methods The AD model rats were established by β-amyloid protein (Aβ 1-42 ) injection into hippocampus CA1 zone. They were assigned into the model group, the donepezil treated group and the GETO treated group, besides, a normal group was set for control. Four weeks after modeling, Morris water maze test was applied to determine the learning and memory function of the AD rats, the number of PSD-95 and Shank-1 protein positive neuron as well as the optical density (OD) in postsynaptic dense zone of hippocampus CA1 area were determined by using immuno-histochemical stain and computerized graphic analysis techniques. Results Morris water maze test showed that the mean escape latent period (MELP) of the model rats obviously prolonged than that of the normal rats, and the times of traversing flat roof obviously decreased (P<0.01), while in the model rats after being treated by GETO, the two parameters were significantly shortened and increased respectively (P<0.01), reaching the level insignificantly different to those in the donepezil group and the normal group. Immunohistochemical test showed that the number of positive stained neuron of PSD-95 and Shank-1 in hippocampus CA1 zone in the model group was significantly different to those in the normal group (P<0.01), while in the GETO group those indexes were insignificantly different to those in the donepezil group and the normal group (P>0.05), but showed a significant difference when compared with those in the model group (P<0.05). Conclusion GETO can obviously improve the function of learning and memory of AD rats induced by Aβ 1-42 , and the mechanism may be associated with its actions in improving expressions of PSD-95 and Shank-1 protein in hippocampus CA1 zone, and recovering the structure and function of synapse and enhancing its plasticity. |
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