温阳中药复方肝之福逆转肝纤维化的实验研究

作者	单位
王丽春	四川大学华西医院感染性疾病中心人类疾病生物治疗教育部重点实验室
赵连三	四川大学华西医院感染性疾病中心人类疾病生物治疗教育部重点实验室
唐红	四川大学华西医院感染性疾病中心人类疾病生物治疗教育部重点实验室
刘丽	四川大学华西医院感染性疾病中心人类疾病生物治疗教育部重点实验室
刘聪	四川大学华西医院感染性疾病中心人类疾病生物治疗教育部重点实验室
张红英	四川大学华西医院病理科
尧凡	四川大学华西医院中药制剂室

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中文摘要:

目的观察温阳中药复方肝之福对实验性肝纤维化的疗效,并初步探讨其作用机制。方法将59只Wistar大鼠分为正常对照组与造模组,造模组采用皮下注射CCl4及饮用100ml/L乙醇的方法制备出大鼠肝纤维化模型后,随机分为肝之福组、秋水仙碱组及模型对照组,肝之福组予肝之福灌胃,每日1次,秋水仙碱组予秋水仙碱灌胃,每日1次,模型对照组则予等量生理盐水灌胃,疗程均为30天。实验结束后测定血清肝功能、血清肝纤维化指标、肝组织羟脯氨酸(Hyp)含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,并根据改进的炎症活动度及纤维化半定量计分系统(SSS)评分,观察治疗组与模型对照组的组织学变化,同时对肝组织内Ⅰ型、Ⅲ型、Ⅳ型胶原进行染色并定量分析。结果与模型对照组比较,肝之福组ALT明显改善,肝纤维化指标(PCⅢ、LN)显著下降,Hyp含量和MDA含量明显降低,而SOD活性则升高,同时SSS评分炎症活动度及肝纤维化程度明显改善,肝组织内Ⅰ型、Ⅲ型、Ⅳ型胶原含量下降,肝之福抗肝纤维化的疗效与秋水仙碱相当。结论肝之福对实验性肝纤维化有减轻或逆转其肝纤维化的作用,抗脂质过氧化是其可能的作用机制。

英文摘要:

Objective To observe the effect of Ganzhifu (GZF), a Chinese compound formula for Warming-yang on experimental hepatic fibrosis, and to explore its mechanism. Methods Fifty-nine Wistar rats were divided into the normal control group and the model group. After the hepatic fibrosis model was established in rats by subcutaneous injection of CCl4 and feeding 10% alcohol in the model group, the rats were randomly sub-divided into the Ganzhifu group, colchicine group and model group, they were treated with Ganzhifu, colchicine and normal saline of same volume once a day respectively for 30 days as one treatment course. The serum liver function and hepatic fibrosis indexes, liver tissue contents of hydroxyproline (Hyp), malondialdehyde (MDA) and superoxide dismutase (SOD) activity were measured at the end of the experiment. Histologic changes of liver tissue were observed and scored by the modified inflammatory activity and fibrosis semiquantative scoring system (SSS), and collagen type Ⅰ,Ⅲ and Ⅳ in liver tissue were stained and semiquantitive analysis conducted. Results In the Ganzhifu group, liver function and inflammatory activity and fibrosis SSS scores were markedly improved, indexes of hepatic fibrosis, liver contents of Hyp, MDA and collagen type Ⅰ,Ⅲ and Ⅳ were significantly decreased, and the SOD activity was increased, the changes were more significant as compared with those in the model group. The protective effect of Ganzhifu against hepatic fibrosis was the same as that of colchicine. Conclusion Ganzhifu can alleviate or reverse hepatic fibrosis in experimental hepatic fibrosis, and antioxidation may be its mechanism.

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