健胃愈疡颗粒对胃溃疡复发大鼠胃组织核因子-κBmRNA及其蛋白表达的影响

Nuclear Factor-κB mRNA and Protein Expression in Stomach Tissue of Rats with Gastric Ulc er Recurrence and Effect of Jianwei Yuyang Granule on Its Expression

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中文关键词: 胃溃疡复发核因子-κB 炎症反应健胃愈疡颗粒

英文关键词:gastric ulcer recurre nce nuclear factor-κB inflammatory reaction Jianwei Yuyang granule

基金项目:湖南省科技计划重点项目(No.02SSY1005-4)

作者	单位
凌江红	中南大学湘雅医院中西医结合研究所长沙410008
李家邦	中南大学湘雅医院中西医结合研究所长沙410008
申定珠	中南大学湘雅医院中西医结合研究所长沙410008
周兵	中南大学湘雅医院中西医结合研究所长沙410008

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中文摘要:

目的观察胃溃疡复发大鼠胃组织的炎症反应、核因子-κB(nuclear factor-κB,NF-κB)mRNA及其蛋白表达和健胃愈疡颗粒的干预作用。方法先后复制大鼠乙酸性胃溃疡及其复发模型,设假手术组、模型组、奥美拉唑组、健胃愈疡组。观察各组大鼠胃组织慢性炎症细胞和中性粒细胞计数、NF-κB mRNA及其蛋白表达的情况。结果灌胃16天和92天时,模型组大鼠胃组织慢性炎症细胞和中性粒细胞计数、NF-κB mRNA及其蛋白表达均较假手术组显著增加(P<0.01),健胃愈疡组慢性炎症细胞和中性粒细胞计数、NF-κB mRNA及其蛋白表达均显著低于模型组(P<0.05或P<0.01),与奥美拉唑组比较差异无显著性。NF-κB mRNA及其蛋白表达均分别与慢性炎症细胞和中性粒细胞计数密切相关(P<0.01)。结论NF-κB可能在调节大鼠乙酸性胃溃疡愈合及其复发的炎症反应中发挥重要作用。健胃愈疡颗粒可能通过抑制胃组织NF-κB的活化和表达抑制炎症反应,这可能是其抗溃疡复发机制之一。

英文摘要:

ObjectiveTo observe the inflammatory reacti on, nuclear fac tor-κB (NF-κB) mRNA and protein expression in stomach tissue of rats wit h gastric ulcer recurrence and the effect of Jianwei Yuyang granule (JYG) o n them. MethodsGastric ulcer and its recurrent lesion were successively induced by acetic acid and interliukin1-β(IL-1β), and t he model rats were divided into the sham operation group,the model group ,the omepraxole group and the JYG group to observe the state of chronic inflammatory cell, neutrophil count, NF-κBmRNA and protein expressio n in stomach tissue. Results On the 16th and 92th day after a dministration, the increase of chronic inflammatory cell, neutrophil, NF-κB m RNA and protein expression in the model group was more significant than those in the sham operated group (P<0.01), while t hat was lower in the JYG group than in the model group (P<0.05, P <0.01), but with no remarkable d ifference to the omepraxole group. In addition, the mRNA and prote in expression of NF-κB were correlated closely with the count of chro n ic inflammatory cell and neutrophil respectively (P<0.0 1). ConclusionN F-κB may play an important role in regulating inflammatory reaction d u ring the healing and recurrence processes of gastric ulcer induced by acetic acid. JYG may suppress inflammatory reaction by inhibiting the activation and expression of NF-κB in stomach tissue, which m ay be one of the mechanisms of JYG in preventing the recurrence of gastri c ulcer.

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