制首乌对脑内灌流6-羟基多巴胺所致大鼠纹状体细胞外液羟自由基升高的影响

Effect of Prepared Radix Polygoni Multiflori on the Elevatio n of Extracellular Hydroxyl Radical in Striatum of Rats Induced by Intracerebral Perfusion of 6-hydroxy Dopamine

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DOI：

中文关键词: 制首乌脑内微透析 6-羟基多巴胺羟自由基水杨酸捕获

英文关键词:prepared Radix Polygoni multiflori cerebral m icrodialysis hydroxyl radical 6-hydroxy dopamine capture by salicylic acid

基金项目:国家自然科学基金资助项目(No.30371824)

作者	单位
王丹巧	中国中医科学院西苑医院北京100091
王巍	中国中医科学院西苑医院北京100091
赵德忠	中国中医科学院西苑医院北京100091

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中文摘要:

目的观察制首乌对脑内灌流6-羟基多巴胺(6-OHDA)所致大鼠纹状体细胞外液羟自由基升高的影响,探讨制首乌对脑保护的作用机理。方法用脑内微透析造模,水杨酸捕获羟自由基,高效液相-电化学检测器测定活体脑内羟自由基所形成的2,3二羟基苯甲酸(2,3-DHBA)和2,5二羟基苯甲酸(2,5-DH-BA)。结果6-OHDA脑内灌流后,模型组大鼠纹状体细胞外液2,3-DHBA和2,5-DHBA均迅速增高,前者在观察全程中一直显著高于对照组(P<0.01);后者部分时间点显著高于对照组(P<0.05或P<0.01);制首乌组的2,3-DHBA有5个时点显著低于模型组(P<0.05或P<0.01)。结论制首乌对6-OHDA脑内灌流引起的细胞外液羟自由基升高有一定程度的抑制作用,提示其脑保护作用的机理之一与抗氧化有关。

英文摘要:

ObjectiveTo investigate the effect and mech anism of prepared Radix Polygoni Multiflori (RPM) on the elevation of extracell ular hydroxyl radical in striatum of rats induced by intracerebral perfusion of 6-hydroxy dopamine (6-OHDA). MethodsCerebral microdia lysis was used to establish the model. Hydroxyl radical was captured by salicylic acid, and 2,3-d i hydroxy benzyl acid (2,3-DHBA) and 2,5-dihydroxy benzyl acid (2,5-DHBA) forme d b y hydroxyl radical in vital brain were measured by high performance liqui d chromatography-electrochemical detector (HPLC-ED). Results After perfu sion of 6-OHDA in brain of rats, the levels of 2,3-DHBA and 2,5-DHBA in the mod el group increased rapidly. The former was higher during the whole course of obs ervation (P<0.01), while the latter was higher at most time points than t hat in the control group (P<0.05 or P< 0.01). The level of 2,3-DHBA in the RPM group was lower than that in the model group at 5 time points (P <0.05, P<0.01). ConclusionRPM could inhibit th e elevating of extracellular hydro xyl radical in striatum of rats induced by intracerebral perfusion of 6-OHDA, i n dicating one of the brain protective mechanisms of RPM may be related to its anti-oxidation effect.

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