脉心康胶囊对载脂蛋白E基因敲除小鼠肝细胞内Ca<sup>2+</sup>及线粒体膜电位的影响

作者	单位
郑广娟	山东中医药大学基础医学院济南250014
张文高	山东中医药大学基础医学院济南250014
朱庆均	山东中医药大学基础医学院济南250014
杨勇	山东中医药大学基础医学院济南250014
张丹	山东中医药大学基础医学院济南250014
王显刚	山东中医药大学基础医学院济南250014
季旭明	山东中医药大学基础医学院济南250014

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中文摘要:

目的观察脉心康胶囊对载脂蛋白E基因敲除〔ApoE(-/-)〕小鼠肝细胞内Ca2+浓度及线粒体膜电位的影响。方法采用胶原酶消化法分离ApoE(-/-)小鼠肝细胞,体外原代培养8天后,加入10%含药大鼠血清的培养液,48h后以Flou-3和Jc-1为探针,应用激光共聚焦显微镜测量肝细胞内Ca2+浓度和线粒体膜电位。结果脉心康可明显降低ApoE(-/-)小鼠肝细胞内Ca2+浓度,与生理盐水及洛代他汀组比较差异有显著性(P<0·01);同时,脉心康可明显提高ApoE(-/-)小鼠肝细胞线粒体膜电位(P<0·01),脉心康的这两种作用无明显的剂量效应关系。结论脉心康可降低ApoE(-/-)小鼠肝细胞内Ca2+浓度,提高线粒体膜电位,从而调节脂代谢、对抗高脂血症及动脉粥样硬化的发生和发展。

英文摘要:

ObjectiveTo observe the effect of Maixinkang Capsule (MXK) on Ca~ 2+ concentration and mitochondrial membrane potential in liver cells of ApoE(-/-) mice. MethodsLiver cells from ApoE(-/-) mice were separated using collagenase digestive method. After the primary cells were cultured for 8 days in vitro, the concentration of 10% MXK contained rat’s serum was added into the culture fluid. The Ca~ 2+ concentration and mitochondrial membrane potential in liver cells after 48-hr culture were measured by confocal laser scanning microscopy with Flou-3 and Jc-1 as probes. ResultsMXK could decrease Ca~ 2+ concentration in liver cells, which was significantly different to that in the control group (P<0.01). Meanwhile, MXK could significantly improve mitochondrial membrane potential in liver cells (P<0.01). There was no obvious dose-effect relationship shown in both effects of MXK. Conclusion MXK can decrease Ca~ 2+ concentration and improve the mitochondrial membrane potential in liver cells of ApoE(-/-) mice so as to regulate the lipids and prevent the occurrence and development of hyperlipemia and atherosclerosis.

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