二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究

Experimental Study on Effect of Er’zhi Tiangui Granule in Improving Quality of Oocyte and Its Correlation with Level of Insulin-like Growth Factor-1R mRNA Expression in Ovary of Mice

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中文关键词: 二至天癸颗粒卵细胞质量卵裂率颗粒细胞胰岛素样生长因子-1受体

英文关键词:Er’zhi Tiangui Granule quality of oocyte cleavage rate granular cell insulin-like growth factor-1 receptor

基金项目:国家自然科学基金资助课题(No.90209058)

作者	单位
连方	山东中医药大学附属医院生殖中心
孙振高	山东中医药大学附属医院生殖中心
穆琳	山东中医药大学
张建伟	山东中医药大学
刘延荷	山东中医药大学附属医院生殖中心
张宁	山东中医药大学
孟茜	山东中医药大学附属医院生殖中心
马凤梅	山东中医药大学附属医院生殖中心
滕依丽	山东中医药大学
刘海萍	山东中医药大学

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中文摘要:

目的探讨二至天癸颗粒提高卵细胞质量的机理。方法90只小鼠分为6组,实验1组与对照1组观察排卵后优质卵数目。实验2组与对照2组观察鼠胚卵裂率。用原位杂交的方法对实验3组与对照3组鼠卵颗粒细胞IGF-1R mRNA表达量进行检验。结果实验组与对照组优质卵率分别为〔(78±8)%、(71±5)%〕,卵裂率分别为〔(88±3)%、(83±5)%〕及颗粒细胞IGF-1R mRNA表达量分别为〔(0·4890±0·0454)、(0·4439±0·0283)〕,两组比较差异有显著性(P<0·05)。优质卵率与颗粒细胞IGF-1R mRNA表达量呈正相关。结论二至天癸颗粒提高卵细胞质量的机理可能与提高颗粒细胞IGF-1R mRNA的表达量有关。

英文摘要:

ObjectiveTo explore the mechanism of Er’zhi Tiangui Granule (ETG) in improving the quality of oocyte. MethodsNinety mice were randomly divided into 6 groups.The number of high-quality oocytes was comparatively observed in the 1st experimental group and the 1st control group; the embryonic cleavage rate was observed in the 2nd experimental group and the 2nd control group and the quantity of insulin-like growth factor-1R mRNA (IGF-1R mRNA) expression in ovarian granular cells was determined by using in situ hybridization in the 3rd experimental group and the 3rd control group. ResultsThe high-quality oocytes rate, the embryonic cleavage rate and the quantity of IGF-1R mRNA expression in the three paired groups was (78±8)% vs (71±5)%, (88±3)% vs (83±5)%, 0.4890±0.0454 vs 0.4439±0.0283, respectively. The difference between the experimental groups to the respective control groups was significant (all P<0.05), and positive correlation was shown between the high-quality oocytes rate and the quantity of IGF-1R mRNA expression. ConclusionThe mechanism of ETG in improving the quality of oocyte may be related with the elevation of IGF-1R mRNA level in ovarian granular cells.

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