姜酚对中暑内毒素血症模型小鼠的影响

作者	单位
聂红	暨南大学药学院药理教研室
孟兰贞	暨南大学药学院药理教研室
张慧	暨南大学药学院药理教研室
钟玲	暨南大学药学院实验技术中心
黄雪松	暨南大学理工学院食品科学与工程系广州510632

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中文摘要:

目的观察姜酚在中暑内毒素血症发病过程中所起的作用。方法将小鼠随机分为生理盐水组、中暑内毒素血症模型组、常温对照组、姜酚组和溶剂对照组,每组8只。除常温对照组暴露于室温(25±0．5)℃、相对湿度(43±5)％条件下2 h外,其余各组均在人工热气候模拟舱干球温度(35±0．5)℃、相对湿度(65±5)％的条件下进行热暴露2 h,诱导其产生中暑内毒素血症,除模型组外,其余各组于造模前分别给予姜酚、溶剂蔗糖酯、生理盐水。造模后采用MTT法测定小鼠腹腔巨噬细胞的能量代谢水平,中性红比色法检测巨噬细胞的吞噬功能,黄嘌呤氧化酶法检测血浆SOD活性,TBA法检测血浆MDA含量,用透射电镜观察肝细胞的超微结构。结果姜酚组腹腔巨噬细胞的能量代谢水平、吞噬能力和血浆SOD活性均显著高于模型组(P<0．01),血浆MDA含量则低于模型组(P<0．01),血浆SOD活性和MDA含量与常温对照组比较无显著差异(P>0．05);中暑内毒素血症模型组部分肝细胞核变形、不规则,线粒体肿胀,线粒体嵴减少,溶酶体增多,糖原颗粒减少,出现大量脂滴;姜酚组可显著改善中暑所致的内毒素血症小鼠肝细胞超微结构的脂肪样病变。结论姜酚通过缓解中暑对肝脏的损伤,提高中暑内毒素血症模型小鼠腹腔巨噬细胞能量代谢水平,增强其吞噬能力,增加血浆SOD活性,减少MDA生成,对小鼠有保护作用。

英文摘要:

Objective To observe the effect of Gingerol on endotoxemia mouse induced by heatstroke. Methods Forty mice were randomly divided into five groups, the endotoxemia model group (A), the normal temperature group (B), the Gingerol treated group (C), the solvent control group (D), and the saline control group (E), 8 mice in each group. Group B to E was administered with saline, Gingerol, solvent and saline respectively. Mice in group B were placed at room temperature 25±0.5℃, relative humidity 43±5% for 2 hrs, while mice in the other groups were exposed under 35±0.5℃and relative humidity 65±5% for 2 hrs in an artificial hot-climate mimic cabin to establish heatstroke endotoxemia model. The energy metabolic level of ccliomacrophage was detected with MTT; the phagocytic ability was examined with neutral red chromometry; the hepatocyte ultrastructure was observed with transmission electron microscopy, as well as the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in plasma was tested. Results As compared with Group A, D and E, in Group C, energy metabolic levels of macrophage, phagocytic ability, and activity of SOD were significantly higher (P<0.01), and the level of MDA was significantly lower respectively (P< 0.01), with the levels of SOD and MDA approaching to those in Group B (P>0.05). The pathologic changes of hepatocyte ultrastructure in group C were less than those in the other three endotoxemia groups. Conclusion Gingerol could raise the energy metabolic level of celio-macrophage to enhance its phagocytic ability, increase the activity of SOD and reduce the production of MDA in mouse with heatstroke endotoxemia, so as to alleviate the liver damage.

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