戴高中,陈汝兴,卫洪昌.大黄虫丸对大鼠脑出血模型海马组织氨基酸含量的影响[J].中国中西医结合杂志,2006,(6):538-540 |
大黄虫丸对大鼠脑出血模型海马组织氨基酸含量的影响 |
Effect of Dahuang Zhechong Pill on Content of Amino Acids in Hippocampal Tissue of Ischemic Cerebral Hemorrhagic Rats |
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DOI: |
中文关键词: 脑出血 大黄虫丸 兴奋性氨基酸 抑制性氨基酸 |
英文关键词:cerebral hemorrhage Dahuang Zhechong Pill excitatory amino acid inhibitory amino acid |
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中文摘要: |
目的观察大黄丸对大鼠脑出血模型海马组织氨基酸含量的影响,探讨大黄虫丸对脑出血大鼠保护作用的可能机制。方法选择胶原酶加肝素联合注射法诱导脑出血大鼠模型,采用反相高效液相色谱荧光法观察大黄虫丸对大鼠脑组织海马中谷氨酸(glutamic acid,Glu)、天门冬氨酸(aspartic acid, Asp)及γ-氨基丁酸(γ-aminobutyric acid,GABA)的影响。结果脑出血模型组3天后海马的氨基酸含量(μmol/g)Glu(15.2926±4.2429)、Asp(3.6384±3.1021)和GABA(0.3859±0.1846)含量明显的高于正常组Glu(8.5040±1.7794)、Asp(1.2669±0.4695)和GABA(0.1770±0.0472)和假手术组Glu(8.0057±1.1227)、Asp(1.4986±0.4174)和GABA(0.1829±0.04665)(P<0.05),而中药大黄虫丸治疗组的Glu (9.0550±1.7195)、Asp(1.8085±0.6862)和GABA(0.1993±0.0424)含量明显降低于脑出血模型组(P< 0.05)。结论提示大黄虫丸能抑制兴奋性氨基酸(Glu、Asp)的释放,纠正兴奋性氨基酸(EAA)/抑制性氨基酸(IAA)失衡,从而达到减轻脑组织损害的作用。 |
英文摘要: |
Objective To observe the effect of Dahuang Zhechong Pill (DZP) on the content of amino acids in hippocampal tissue of model rats of ischemic cerebral hemorrhage (ICH). Methods Collagenase and heparin were injected into caudate nucleus to establish ICH rat model. The content of glutamic acid (Glu), aspartic acid (Asp) andγ-aminobutyric acid (GABA) in hippocampal tissue was detected by anti-phase high-performance liquid chromatographic fluorimetry to observe the effect of DZP on the content of amino acids in brain tissue. Results Three days after modeling, the content of Glu, Asp and GABA was significantly higher in the model group than those in the sham-operation and the normal control group (P<0.05), for Glu 15.2926±4.2429μmol/g vs 8.0057±1.1227μmol/g and 8.5040±1.7794μmol/g, for Asp 3.6384±3.1021μmol/g vs 1.4986±0.4174μmol/g and 1.2669±0.4695μmol/g, and for GABA 0.3859±0.1846μmol/g vs 0.1829±0.04665μmol/g and 0.1770±0.0472μmol/g. The content of Glu (9.0550±1.7195μmol/g), Asp (1.8085±0.6862μmol/g) and GABA (0.1993±0.0424μmol/g) in hippocampal tissue in the DZP group was reduced significantly, as compared with that in the model group (P<0.05). Conclusion DZP could reduce the release of excitatory amino acids (EAA-Glu, ASP) to balance EAA/ inhibitory amino acid (IAA), so as to relieve ICH-in-duced brain injury. |
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