通心络对大鼠心肌梗死后心肌炎症反应及肿瘤坏死因子-α表达的影响

作者	单位
孔强	都医科大学附属朝阳医院心脏中心首都医科大学心血管病研究所
杨新春	都医科大学附属朝阳医院心脏中心首都医科大学心血管病研究所
刘秀兰	都医科大学附属朝阳医院心脏中心首都医科大学心血管病研究所
吕月平	首都医科大学附属朝阳医院基础实验中心北京 100020
石亮	首都医科大学附属朝阳医院基础实验中心北京 100020

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中文摘要:

目的研究通心络对大鼠心肌梗死后心肌炎症反应及肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)表达的影响。方法应用结扎冠脉前降支的方法制备大鼠心梗模型,将SD大鼠随机分为3组:假手术组、心梗组、通心络干预组[2．0g／(kg·d)],应用组织病理学的方法观察术后1、2及4周梗死区与非梗死区炎性细胞浸润的情况;以半定量RT-PCR及蛋白免疫印迹(Western blot)的方法检测炎性因子TNF-α的表达情况。结果心肌梗死后1周及2周,在心梗组及通心络干预组,梗死区与非梗死区炎性细胞浸润明显,至心肌梗死后4周,炎症反应消退,但TNF-α表达持续增加。通心络干预后炎性细胞浸润减轻,TNF-α表达降低。结论通心络能减轻心梗后心肌炎症反应,降低炎性因子TNF-α的表达,发挥抗炎作用。

英文摘要:

Objective To investigate the effects of Tongxinluo (TXL) on myocardial inflammatory reaction and expression of tumor necrosis factor-α(TNF-α) in rats with myocardial infarction (MI). Methods The rat MI model was established by left anterior descending coronary artery ligation. Experimental rats were randomly divided into 3 groups: the sham operated group, the MI control group and the TXL group (treated by 2.0 g/ kg/day). Conditions of inflammatory cellular infiltration in the infarcted and non-infarcted zone were observed at 1, 2 and 4 weeks after MI with histopathological methods, and the expression of TNF-αwas detected by semi-quantitative RT-PCR and Western blot. Results Obvious inflammatory cellular infiltration revealed in both infarcted and non-infarcted zone of model rats at 1-2 weeks after MI, it faded away gradually 4 weeks after MI, while the TNF-αexpression increased continuously after MI. Compared with the MI control, the infiltration was milder and TNF-αexpression was lower in the TXL group. Conclusion TXL displays its anti-inflammatory action by way of attenuating the myocardial inflammatory reaction and suppressing the expression of inflammatory cytokine TNF-αin MI rats.

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