慢性阻塞性肺疾病肺气虚证患者T淋巴细胞差异表达基因的初步研究

Preliminary Study on Differential Expressed Genes in T Lymphocyte of Chronic Obstructive Pulmonary Disease Patients with Fei-qi Deficiency Syndrome

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中文关键词: 慢性阻塞性肺疾病肺气虚证 T淋巴细胞基因表达基因芯片

英文关键词:chronic obstructive pulmonary disease Fei-qi deficiency syndrome T lymphocyte gene expression gene chips

基金项目:国家中医药管理局科学技术研究专项课题(No.02-03JL04)

作者	单位
李泽庚	安徽中医学院第一附属医院呼吸内科
童佳兵	安徽中医学院第一附属医院呼吸内科
彭波	安徽中医学院第一附属医院呼吸内科
张念志	安徽中医学院第一附属医院呼吸内科
季红燕	安徽中医学院第一附属医院呼吸内科
郑汉城	中国科学院上海生命科学院健康研究所

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中文摘要:

目的通过基因芯片技术研究慢性阻塞性肺疾病(COPD)肺气虚证患者T淋巴细胞相关差异基因表达情况。方法采集肺气虚证和肺阴虚证患者、健康人的外周血作为实验血样和对照血样。采用Ficoll技术分离外周血淋巴细胞、流式细胞仪分选并纯化T淋巴细胞,采用人类全基因表达谱芯片技术筛选出差异表达基因。结果肺气虚证组/肺阴虚证组和肺气虚证组/健康对照组均高表达的差异基因15条。结论基因芯片技术能有效地研究中医证的基因表达谱并初步筛查出COPD肺气虚证患者T淋巴细胞相关差异表达基因。

英文摘要:

ObjectiveTo study T lymphocyte related genes with differential expression in patients with chronic obstructive pulmonary disease(COPD)of Fei-qi deficiency(FQD)syndrome type by gene chips.Methods Lymphocytes in peripheral blood were isolated by Ficoll technique from blood samples collected from COPD patients of FQD syndrome type,Fei-yin deficiency(FYD)syndrome type,and also from healthy subjects for control.They were sorted and purified by flow cytometry,and the different expressed genes were screened from them by gene chip technique.Results There were 15 genes with high differential expression between patients of FQD type and those of FYD syndrome type,and between patients of FQD type and healthy subjects.Conclusion Gene chip technique could be used for studying the gene expression profiles of TCM syndrome,and the T-lymphocyte related genes with differential expression in COPD patients with FQD were screened preliminarily.

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