三氧化二砷对BXSB狼疮小鼠自身免疫及其生存时间的影响

作者	单位
夏晓茹	温州医学院附属第一医院风湿免疫科 (浙江325000)
林素仙	温州医学院附属第一医院风湿免疫科 (浙江325000)
周艳	温州医学院附属第一医院风湿免疫科 (浙江325000)
朱小春	温州医学院附属第一医院风湿免疫科 (浙江325000)
高申孟	温州医学院附属第一医院风湿免疫科 (浙江325000)
许菲菲	温州医学院附属第一医院风湿免疫科 (浙江325000)

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中文摘要:

目的探讨三氧化二砷(Arsenic Trioxide,ATO)对BXSB狼疮小鼠自身免疫及其生存时间的影响。方法BXSB狼疮小鼠随机平分两组,即ATO治疗组和对照组,ATO0.4mg/(kg.d)隔日腹腔注射至90天和105天,观察至210天实验结束,比较两组小鼠生存时间的差异;采用ELISA法检测小鼠血清抗ds-DNA抗体和IgG表达水平,RT-PCR法分别检测脾和肾组织干扰素-γ(interferon gamma,IFN-γ)mRNA表达水平。结果两组BXSB小鼠(每组各17只)生存时间比较,至210天时,ATO治疗组总死亡8只,而对照组总死亡13只,两组比较差异有显著性(χ2=4.20,P<0.05);血清抗ds-DNA抗体(OD值)对照组为0.566±0.080,ATO组为0.392±0.087,低于对照组(P<0.001)。ATO组105天的血清IgG水平较治疗前和对照组均明显下降(P<0.05)。治疗组与对照组小鼠脾组织IFN-γmRNA表达(灰度比值)分别为0.540±0.300和2.320±0.522(P<0.01);肾组织IFN-γmRNA表达(灰度比值)分别为0.338±0.163和0.588±0.104(P<0.05)。结论ATO能够延长BXSB小鼠的生存时间,降低外周血抗ds-DNA抗体和IgG的表达水平,抑制脾和肾组织IFN-γmRNA的高表达。

英文摘要:

ObjectiveTo investigate the effect of arsenic trioxide(ATO)on the autoimmunity and survival time in BXSB lupus mice.MethodsThe model BXSB lupus mice were randomly divided into two groups equally,the ATO treated group and the control group,17 in each group.Mice in the ATO group were given intraperitoneal injection of ATO at the daily dose of 0.4 mg/kg,once every other day for 105 days or 90 days,respectively,and the observation lasted for 210 days.The survival time between the two groups was compared;the serum levels of anti-dsDNA and IgG were detected by enzyme-linked immunosorbent assay(ELISA),and the interferon-γ(IFN-γ)mRNA expression in renal and spleen tissue were measured by reverse transcriptase polymerase chain reaction(RT-PCR)technique.ResultsTill the 210th day,the total number of death was 8 in the ATO treated group and 13 in the control group,comparison between the two groups showed significantly different(χ2=4.20,P<0.05).The mean OD value of serum anti-dsDNA antibody was lower in the ATO group(0.392 ± 0.087)than that in the control group(0.566±0.080,P<0.001).The serum level of IgG on day 105 in the ATO group was significantly lower than that in the control group and before treatment(P<0.05).The expression of IFN-γ mRNA in spleen tissue and renal tissue in the ATO group and the control group was 0.540±0.300 and 0.338±0.163,2.320±0.522 and 0.588±0.104(P<0.05 and P<0.01 respectively).ConclusionATO can prolong the survival time of BXSB lupus mice,decrease the peripheral level of anti-dsDNA antibody and IgG expression,inhibit the over-expression of IFN-γ mRNA in spleen and kidney tissues.

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