益气化瘀补肾方及拆方影响椎间盘细胞外基质胶原和代谢酶mRNA表达的研究

Study on Yiqi Huayu Bushen Recipe and Its Disassembled Recipes in Regulating mRNA Expression of Collagens and Metabolic Enzymes in Extracellular Matrix of Cervical Disc

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中文关键词: 益气化瘀补肾方细胞外基质胶原基质金属蛋白酶基质金属蛋白酶组织抑制剂逆转录多聚酶链式反应

英文关键词:Yiqi Huayu Bushen Recipe extracellular matrix collagen matrix metalloproteinase tissue inhibitor of metalloproteinase reverse transcription-polymerase chain reaction

基金项目:国家自然科学基金重点项目(No.30330700);国家自然科学基金面上项目(No.30371794&No.30171170);上海市科技“启明星”跟踪计划(No.05QMH1412);上海市医学重点学科建设项目(No.05Ⅲ027);上海市重点学科建设项目(T0303)

作者	单位
施杞	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
王拥军	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
李晨光	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
周泉	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
胡志俊	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
刘梅	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院
周重建	上海中医药大学脊柱病研究所上海中医药大学附属龙华医院

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中文摘要:

目的探讨益气化瘀补肾方及拆方影响退变模型大鼠颈椎间盘细胞外基质中Ⅰ型、Ⅲ型、Ⅹ型胶原和相关代谢酶MMPs/TI MP mRNA的表达。方法采用RT-PCR法检测胶原和MMPs/TI MP mRNA表达,凝胶成像系统扫描仪对各条带进行扫描,计算机自动计算出光密度。结果与假手术组比较,模型组Ⅰ型、Ⅲ型、Ⅹ型胶原和MMP-13mRNA表达明显升高(P<0.01);TI MP-1mRNA表达无明显统计学差异(P>0.05)。益气化瘀补肾方及其拆方可明显降低Ⅰ型、Ⅲ型、Ⅹ型胶原和MMP-13mRNA的过度表达(P<0.01或P<0.05)。结论退变椎间盘的Ⅰ型、Ⅲ型、Ⅹ型胶原mRNA表达增高,MMP-13mRNA的高表达和TI MP-1mRNA的低表达,MMPs/TI MP的比例失调,反映了退变椎间盘ECM合成和降解的失调。益气化瘀补肾方及拆方可能通过影响颈椎间盘组织胶原及相关代谢酶mRNA表达而延缓椎间盘的退变。

英文摘要:

ObjectiveTo study the effects of Yiqi Huayu Bushen Recipe(YHBR)and its disassembled recipes on mRNA expressions of collagen Ⅰ,Ⅲ,Ⅹ,matrix metalloproteinase(MMPs)and tissue inhibitor of metalloproteinase(TIMP)in extracellular matrix of cervical disc in model rats of cervical vertebral disc degeneration.MethodsThe mRNA expressions of collagens,MMP-13 and TIMP-1 were detected by RT-PCR.The strips were scanned by gel imaging system scanner,and the optical density was autocalculated by computer.ResultsCompared with those of the sham-operative group,the mRNA expressions of collagen Ⅰ,Ⅲ and Ⅹ and MMP-13 of the model rats increased markedly(P<0.01),which were lowered by YHBR and its disassembled recipes(P<0.01 or P<0.05),and the levels after YHBR treatment were significantly different to those after Western medicine treatment.However,no remarkable change was found in TIMP-1 mRNA expression in the model rats(P>0.05).ConclusionIn the degenerated intervertebral disc the mRNA expressions of collagen Ⅰ,Ⅲ,Ⅹ and MMP-13 increased,TIMP-1 mRNA expression decreased and the proportion of MMPs/TIMP was in imbalance.YHBR and its disassembled recipes could postpone the degeneration of intervertebral disc through regulating mRNA expressions of collagens and their correlated metabolic enzymes.

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