| 朱跃科,段钟平,王宝恩,刘芳,赵军,刘道洁,谢贤春.芪参益气滴丸抗大鼠肝纤维化的实验研究[J].中国中西医结合杂志,2007,(3):223-226 |
| 芪参益气滴丸抗大鼠肝纤维化的实验研究 |
| Experimental Study on Effects of Qishen Yiqi Dropping Pills on Liver Fibrosis in Rats |
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| DOI： |
| 中文关键词: 芪参益气滴丸 肝纤维化 Ⅰ、Ⅲ型胶原 1型组织性基质金属蛋白酶抑制物 |
| 英文关键词:Qishen Yiqi Dropping Pills liver fibrosis collagen typeⅠandⅢ tissue inhibitor of metalloproteinase-1 |
| 基金项目:科技部国家科技攻关计划引导项目(No．2003BA753C) |
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| 中文摘要: |
| 目的研究芪参益气滴丸抗肝纤维化的作用机制。方法60只大鼠随机分为正常组、模型组、干预组、对照组及芪参益气滴丸组(简称治疗组)。采用二甲基亚硝胺腹腔内注射建立肝纤维化大鼠模型。按6期分类法评价各组肝纤维化程度。应用RT-PCR检测各组肝组织中Ⅰ、Ⅲ型胶原和1型组织性基质金属蛋白酶抑制物(TIMP-1)mRNA水平;应用免疫组织化学技术检测各组动物肝组织Ⅰ、Ⅲ型胶原和TIMP- 1的表达。结果干预组与治疗组大鼠肝纤维化程度、肝组织中Ⅰ型与Ⅲ型胶原和TIMP-1mRNA水平及肝组织中Ⅰ型与Ⅲ型胶原和TIMP-1的阳性表达均分别较模型组与对照组显著减轻与下降。结论芪参益气滴丸能明显减轻肝组织病理改变和肝纤维化程度,抑制Ⅰ、Ⅲ胶原和TIMP-1的表达是其抗肝纤维化的部分机制。 |
| 英文摘要: |
| Objective To investigate the anti-fibrotic effects of Qishen Yiqi Dropping Pills (QYDP) in rats with liver fibrosis (LF).Methods The LF model was induced by intraperitoneal injection with dimethylni- trosamine (DMN).Sixty Wistar rats were randomly divided into the normal group,the model group A,the QYDP intervened group,the model group B,and the QYDP treated group B.The degree of LF was evaluated according to 6-phase grading method.The expressions of collagen typeⅠandⅢand tissue inhibitor of metallo- proteinase-1 (TIMP-1) in liver tissues were determined by immunohistochemistry and the levels of collagen typeⅠandⅢand TIMP-1 mRNA determined by semi-quantitive RT-PCR.Results Compared with the model group A and B,the degree of LF,the positive expressions of TIMP-1 mRNA and the content of collagen typeⅠandⅢin liver tissue in the QYDP intervened and treated groups were significantly lower.Conclusion QY- DP could reduce the pathological changes and degree of LF in rats,which may be partially through inhibiting the expressions of collagen typeⅠandⅢand TIMP-1. |
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