体外培养海马神经元糖氧剥夺损伤模型的建立及川芎嗪对其胞内钙离子的影响

Establishment of Oxygen and Glucose Deprive Model of in vitro Cultured Hippocampal Neuron and Effect of Ligustrazine on Intracellular Ca²⁺ Level in Model Neurons

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中文关键词: 川芎嗪海马神经元细胞培养脑缺血、缺氧钙离子

英文关键词:ligustrazine hippocampal neuron cell culture brain hypoxia/ischemia calcium

基金项目:国家自然科学基金(No．30371789,30672652);浙江省自然科学基金项目(No．Z204424)

作者	单位
万海同	浙江中医药大学心脑血管病研究所
王玉	浙江中医药大学心脑血管病研究所
杨洁红	浙江中医药大学心脑血管病研究所
朱慧渊	浙江中医药大学心脑血管病研究所
郭莹	浙江中医药大学心脑血管病研究所
严伟民	浙江大学生物医学与仪器科学学院
韩进	浙江中医药大学心脑血管病研究所

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中文摘要:

目的研究川芎嗪对体外培养海马神经元糖氧剥夺损伤细胞内钙离子[Ca²⁺]i的影响。方法建立体外培养海马神经元糖氧剥夺（OGD）模型,应用激光共聚焦显微技术检测不同剂量川芎嗪对OGD状态下海马神经元内Ca²⁺的影响。结果成功建立海马神经元OGD模型,经检测模型组[Ca²⁺]i高于空白对照组（P＜0．05）;阳性药组和川芎嗪大、中剂量组[Ca²⁺]i均低于模型组（P＜0．05）。结论海马神经元OGD存在钙超载,川芎嗪具有钙拮抗作用,对脑组织有保护作用。

英文摘要:

Objective To establish the oxygen and glucose deprive （OGD） model in cultured hippocampal neuron and study the effect of ligustrazine on intracellular Ca²⁺ level in the model neurons.Methods The OGD model was established in cultured hippocampal neuron,and the intraeellular Ca²⁺ level in it was detected by laser scanning confocal microscope （LSCM）.Results The OGD model was successfully established in cultured hip- pocampal neurons;the intracellular Ca²⁺ level in the OGD model group was significantly higher than that in the blank control group （P＜0.05）,and that in the nemodipine and high and medium dosage of ligustrazine treated groups was lower than that in the OGD model group （P＜0.05）.Conclusion Intracellular Ca²⁺ overload oc- curs in OGD model neuron,which could be antagonized by ligustrazine,indicating that ligustrazine has a protec- tive effect on hippocampal neuron from hypoxic-ischemic injury.

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