银杏叶提取物金纳多心停跳液对人心肌细胞bcl-2和bcl-xL基因表达的影响

Effects of Ginkgo biloba Extract (Ginaton) on mRNA Expression of bcl-2 and bcl-xL in Myocardium of Patients Underwent Hypothermic Cardiopulmonary Bypass

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中文关键词: 银杏叶提取物(金纳多) bcl-2 bcl-xL 基因表达

英文关键词:Ginkto biloba extract (Ginaton) bcl-2 bcl-xL gene expression

基金项目:贵州省优秀科技教育人才省长专项资金项目(No．黔省专合字[2005]34);贵州省中医药管理局资助项目(No．2004-1)

作者	单位
邓云坤	贵州省人民医院
韦方	贵州省人民医院
李永念	贵阳医学院免疫教研室
陈静	贵州省人民医院
刚绍鹏	贵州省人民医院

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中文摘要:

目的探讨银杏叶提取物金纳多(简称金纳多)对体外循环人心肌细胞抗凋亡基因bcl-2和bcl- xL表达的影响。方法择期先天性心脏病患儿30例,随机均分为两组,治疗组在灌注St Thomas’停搏液(4℃)中加入金纳多(0．5 mg/kg),心脏完全停跳后开始心内手术;对照组单纯灌注St Thomas’停搏液(4℃)。在手术结束开放升主动脉前取心肌组织,采用RT-PCR技术在凝胶电泳成像基础上半定量检测bcl- 2和bcl-xL基因的表达水平。结果治疗组bcl-2和bcl-xL基因的表达显著高于对照组(P＜0．05)。结论银杏叶提取物金纳多可促进人心肌细胞抗凋亡基因bcl-2和bcl-xL的表达。

英文摘要:

Objective To investigate the effects of Ginkgo biloba extract (Ginaton) on bcl-2 and bcl-xL mRNA expression in the myocardium of patients underwent hypothermic cardiopulmonary bypass (CPB). Methods Thirty congenital heart disease patients were randomly assigned to 2 groups,the control group and the treated group.Patients in both groups received St.Thomas’ cardioplegic solusion via radix aortae,while Gi- naton (0.5mg/kg) was added in the treated group.Cardiac surgery was started after complete heart arrest.My- ocardium was taken before the aorta ascendens was unblocked and mRNA expression of bcl-2 and bcl-xL in the ventricular tissue was detected by RT-PCR.Results The gene expressions of bcl-2 and bcl-xL were significant- ly higher in the treated group than those in the control group (P＜0.05).Conclusion Ginaton could promote the mRNA expressions of the antiapoptotic gene bcl-2 and bcl-xL in myocardium of patients underwent CPB.

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