川芎嗪对成年大鼠脑缺血后神经元前体细胞迁移的影响

作者	单位
邱芬	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
刘勇	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
钱亦华	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
康前雁	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
赵建军	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
田英芳	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
陈新林	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
祁存芳	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061
杨杰	西安交通大学医学院神经科学研究中心人体解剖与组织胚胎学系西安710061

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中文摘要:

目的研究川芎嗪对成年大鼠局灶性脑缺血后神经元前体细胞迁移的影响,初步探讨川芎嗪对脑缺血损伤后功能恢复的作用。方法线栓法制作大鼠左侧大脑中动脉阻塞模型(MCAO),术后2h腹腔注射川芎嗪注射液(40mg/kg,每天1次)、分别于MCAO后3、7、14、21天采用免疫组织化学法观察神经元前体细胞的标志Doublecortin(DCX)在侧脑室室下区(SVZ)及SVZ吻侧迁移流(RMS)的迁移情况。结果缺血3天时,SVZ DCX阳性细胞经RMS迁移至嗅球,持续至21天;缺血7天时,SVZ直接及由RMS向邻近缺血纹状体迁移,14天时明显增加;21天时有少量DCX阳性细胞经胼胝体向缺血皮质迁移。川芎嗪组SVZDCX阳性细胞迁移途径同缺血模型组,但程度明显增强。结论川芎嗪可促进神经元前体细胞直接迁移至缺血皮质和纹状体,提示川芎嗪可能通过促进经元前体细胞的迁移对脑缺血后脑功能的自身恢复起重要作用。

英文摘要:

Objective To study the effect of ligustrazine on the migration of neuronal precursors(NPs) after focal cerebral ischemia in adult rats and explore its acting mechanism on recovery of function.Methods Rat model of left middle cerebral artery occlusion(MCAO) was established by thread ligation.Ligustrazine 40 mg/kg was injected peritoneally once a day 2 h after modeling.On the 3rd,7th,14th and 21st day after operation,the migration of Doublecortin(DCX,the marker of NPs) in subventricular zone(SVZ) and the rostral migratory stream(RMS) were observed with immunohistochemistry.Results The migration of DCX-positive cells in SVZ(abbrev.as migration below) through RMS into the olfactory bulb started from the 3rd day after ischemia,and lasted to the 21st day;the migration directly or through RMS into the ischemic penumbra of the adjacent striatum started on the 7th day,and increased significantly on the 14th day;and a few of DCX positive cells migrated through corpus callosum into the ischemic cortex on the 21st day.The migration was similar in the two groups in its pathway,but the extent in the ligustrazine group was more intensive.Conclusion Ligustrazine could promote direct migration of NPs into the ischemic cerebral cortex and striatum,suggesting that it might play an important role in promoting self-recovery of brain function after ischemia through accelerating the migration of NPs.

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