TFAR-19凋亡相关蛋白在心理应激小鼠胸腺中的变化及加味逍遥丸的调节作用

作者	单位
吴振宇	中国中医科学院广安门医院免疫研究室
张云	中国中医科学院广安门医院免疫研究室
吴广均	中国中医科学院广安门医院免疫研究室
国燕霞	中国中医科学院广安门医院免疫研究室
肖健	北京大学心理系
耿小峰	北京大学心理系
杨均平	江西省中医院

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中文摘要:

目的探讨小站台水环境和电刺激诱发的情绪应激不同时间TFAR-19凋亡相关蛋白和Annex-in-Ⅴ凋亡蛋白的变化与胸腺细胞凋亡的相关性及加味逍遥丸(中药)对其影响。方法分别给小鼠用小站台水环境装置和电刺激条件反射装置诱发心理应激造模;用放射免疫法检测血清糖皮质激素(GC)含量,流式细胞法检测TFAR-19凋亡相关蛋白表达,荧光计数法检测Annexin-Ⅴ凋亡蛋白的百分率。结果小鼠在小站台水环境中,随应激时间的延长,GC含量、TFAR-19蛋白表达率和Annexin-Ⅴ凋亡蛋白率逐渐升高(P<0.05或P<0.01),72h中药组比其对应组TFAR-19蛋白表达率显著升高(P<0.05),Annexin-Ⅴ凋亡率显著下降(P<0.01)。电刺激各组小鼠GC含量、TFAR-19蛋白表达率和Annexin-Ⅴ凋亡蛋白率逐渐升高(P<0.05或P<0.01),5天和7天中药组比其对应组显著下降(P<0.05)。结论小站台水环境是一种慢性持续性应激,电刺激是一种急性、较平缓性应激,这两种应激都可以通过神经-内分泌-免疫网络损伤胸腺功能;加味逍遥丸对两种应激造成的胸腺损伤均有不同程度的保护作用。

英文摘要:

Objective To study the variation of TFAR-19 protein expression and Annexin-Ⅴ apoptosis protein proportion in the mice thymus cell apoptosis procedure induced by water platform environment or electric emotional stress and the regulatory effect of modified Xiaoyao Pill(MXP).Methods The mouse emotional stress model was established by water platform equipment or electrical stimulation.The serum glucocorticoid was detected by radioimmunoassay,the TFAR-19 protein was detected by flow cytometry analysis,and Annexin-Ⅴ apoptosis protein proportion was calculated by immunofluorescence technique.Results In the groups of mouse stressed by water platform environment,the level of serum glucocorticoid,the TFAR-19 protein expression and the Annexin-Ⅴ apoptosis protein proportion increased in the thymus cell along with the stress time prolonging(P<0.05 or P<0.01).The serum glucocorticoid level in mice treated with MXP was lower than that in the untreated group(P<0.05).In the groups of emotional stressed mouse established by electrical stimulation,the above-mentioned variations also revealed.All these variations could be alleviated with MXP(P<0.05).Conclusion The water platform environment stress is a chronic continuous stress and electrical stimulation is an acute smooth stress,both of them could damage thymus function through neuro-endocrineo-immune network,but different in duration for causing severe injury.Chinese medicine MXP can alleviate the damage of thymus induced by either of them to certain degree.

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