| 王丹巧,王巍,景富春,赵晋宁.川芎嗪对帕金森病大鼠脑内灌流左旋多巴引起的脑氧化损伤的作用[J].中国中西医结合杂志,2007,(7):629-632 |
| 川芎嗪对帕金森病大鼠脑内灌流左旋多巴引起的脑氧化损伤的作用 |
| Effects of Tetramethylpyrazine on Brain Oxidative Damage Induced by Intracerebral Perfusion of L-DOPA in Rats with Parkinson’s Disease |
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| DOI： |
| 中文关键词: 川芎嗪 左旋多巴 羟自由基 帕金森病 脑内微透析 |
| 英文关键词:tetramethylpyrazine levodopa hydroxyl radical Parkinson’s disease intracerebral microdialysis |
| 基金项目:国家中医药管理局资助项目(No.04-05ZP65) |
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| 中文摘要: |
| 目的探讨川芎嗪对左旋多巴(L-DOPA)脑内灌流引起的帕金森病(PD)大鼠脑氧化损伤的影响。方法用6-羟基多巴胺(6-OHDA)脑内注射造成PD大鼠模型;采用微透析技术脑内灌流L-DOPA和水杨酸捕获羟自由基,高效液相-电化学检测,动态观察大鼠纹状体细胞外液羟自由基被捕获所形成的2,3-二羟基苯甲酸(2,3-DHBA)、2,5-二羟基苯甲酸(2,5-DHBA)浓度的变化。结果模型组大鼠2,3-DHBA和2,5-DHBA浓度较假手术组分别出现6和7个时间点的显著升高(P<0.05或P<0.01);与模型组比较,川芎嗪大、小剂量组在多个时间点其水平明显降低(P<0.05或P<0.01)。结论川芎嗪可减轻L-DOPA引起的PD大鼠脑氧化损伤。 |
| 英文摘要: |
| Objective To observe the effects of tetramethylpyrazine(TMP)on brain oxidative damage induced by intracerebral perfusion of levodopa(L-DOPA)in rats with Parkinson’s disease(PD).Methods PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine(6-OHDA)and perfused in brain with L-DOPA using microdialysis technique.Changes in levels of 2,3-dihydroxy benzyl acid(2,3-DHBA)and 2,5-dihydroxy benzyl acid(2,5-DHBA)in striatum of rats,formed by extracellular hydroxyl radical from salicylic acid capturing,were dynamically observed at various time points by HPLC-ED.Results After treatment with L-DOPA,2,3-DHBA and 2,5-DHBA in the model group showed significantly higher levels at 6 and 7 time points as compared with those in the sham-operated group at the corresponding time points(P<0.05 or P<0.01),while these abnormal elevations were significantly inhibited in the TMP treated groups,either in large or small dose(P<0.05 or P<0.01).Conclusion TMP could reduce the L-DOPA induced brain oxidative damage in PD rats. |
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