乳腺丸Ⅰ号治疗兔乳腺增生的实验研究

作者	单位
陈晰	哈尔滨医科大学附属二院普外科哈尔滨150086
张建国	哈尔滨医科大学附属二院普外科哈尔滨150086
刘旸	哈尔滨医科大学附属二院普外科哈尔滨150086
高峰	哈尔滨医科大学附属二院普外科哈尔滨150086
单世光	哈尔滨医科大学附属二院普外科哈尔滨150086

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中文摘要:

目的观察乳腺丸Ⅰ号(RXPⅠ)对兔乳腺增生的治疗效果并探讨其机理。方法将60只日本大耳白兔随机分为RXPⅠ高、中、低剂量组,逍遥丸组,模型组及正常对照组,每组10只,前5组予肌肉注射己烯雌酚、黄体酮1个月建立兔乳腺增生模型。之后行相应药物灌胃给药,分别取给药前、给药3个月后及停药3个月后作为3个时间观测点。光、电镜下观察乳腺形态学变化;放射免疫法监测血清雌、孕激素水平;免疫组化法测定乳腺组织雌、孕激素受体(ER、PR)表达情况。结果与给药前及模型组比较,高、中剂量RXPⅠ组治疗后增生乳腺小叶腺泡数目、导管腺上皮细胞层数、结缔组织和毛细血管数量均明显减少,乳腺上皮细胞细胞器数量减少(均P<0.05),部分增生细胞凋亡;血清雌二醇(E2)含量显著降低(P<0.01),孕酮(P)升高(P<0.05);乳腺组织ER的表达下调(P<0.05),PR表达无明显变化(P>0.05)。停药3个月后,各监测指标保持平稳,无反弹。结论RXPⅠ治疗具有减轻乳腺组织增生病变、降低E2水平及下调ER表达的作用,对兔乳腺增生具有显著的治疗作用。

英文摘要:

Objective To observe the effect of Ruxian PillⅠ(RXPⅠ) on mammary gland hyperplasia (MGH) in rabbits and to explore its mechanism. Methods Sixty rabbits were divided into the high, medium and low dose of RXPⅠgroups, the Xiaoyao Pill (XYP) group, the model group and the normal control group with 10 in each group. The former 5 groups were injected with diethylstilbestrol and progesterone intramuscularly for one month to induce the MGH model and then given respective medicines via gastrogavage for 3 months. The changes in morphology of mammary gland were observed using light and electronic microscope, the levels of estradiol (E2) and progestogen (P) were measured by radioimmunoassay, and the expression of estradiol receptor (ER) and progestogen receptor (PR) were detected with immunohistochemistry before, at the end of and 3 months after the treatment. Results Compared with those before treatment and those in the model group, in the high and midium dose of RXPⅠgroups after treatment, obvious decrease of acini number in hyperplastic lobuli mammae, connective tissues and blood capillaries, layers of glandular epithelium cells and organellers were seen with partial of hyperplastic cell apoptosis in them. Besides, the serum E2 level decreased obviously (P<0.05), while the serum P level increased, and the ER expression down-regulated significantly (P<0.05), but no obvious changes of PR expression was found. Three months later, all the above indexes maintained stable without rebound. Conclusion RXPⅠtreatment could alleviate the hyperplasia of mammary glands, reduce E2 level, and down-regulate ER expression in rabbits with MGH, showing a significant therapeutical effect.

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