周国钰,胡学强,陈晓红,苏兴文,邱鹏新.步长脑心通含药血清抗缺氧诱导的皮质神经元凋亡的实验研究[J].中国中西医结合杂志,2007,(9):835-838 |
步长脑心通含药血清抗缺氧诱导的皮质神经元凋亡的实验研究 |
Experimental Study on Effect of Buchang Naoxintong Containing Serum for Antagonizing Hypoxia-induced Apoptosis of Cortical Neurons |
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DOI: |
中文关键词: 步长脑心通 神经保护 凋亡 中药血清药理学 皮质神经元 |
英文关键词:Buchang Naoxintong neuroprotection apoptosis sero-pharmacology of Chinese drugs cortical neuron |
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中文摘要: |
目的运用中药血清药理学方法研究步长脑心通抗缺氧诱导的原代皮质神经元凋亡的作用。方法培养出生24h内SD乳鼠大脑皮质神经元,第7天以不同浓度步长脑心通含药血清处理后制造缺氧模型,定性定量检测细胞凋亡:MTT法观察神经元活力;Hoechst33258染色分析细胞核形态的变化;琼脂糖凝胶电泳分析DNA断裂,进一步验证和检测细胞凋亡。结果步长脑心通含药血清处理后缺氧神经元活力增加,缺氧所致神经元凋亡明显减少。结论步长脑心通对缺氧引起的皮质神经元凋亡有抑制作用。 |
英文摘要: |
Objective To investigate the effect of Buchang Naoxintong containing serum (BNCS) for antagonizing hypoxia-induced apoptosis of primary cultured cortical neurons with the sero-pharmacological method. Methods Primary cortical neurons from neonate rats (within 24 h) were cultured and induced into hypoxia model on the 7th day, which were then treated with different concentrations of BNCS. Cell apoptosis was detected qualitatively and quantitatively; and further verified by agarose gel electrophoresis through analyzing internucleosomal DNA fragmentation of the neurons. Besides, neuron viability was tested by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and change of nuclear morphology was observed by Hoechst 33258 staining. Results After treatment with BNCS, the viability of the hypoxia neurons improved with significantly reduced neuron apoptosis. Conclusion Buchang Naoxintong can protect cortical neurons from hypoxia-induced apoptosis. |
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