泻火解毒、消瘀除痰法对脑出血大鼠钙及细胞间黏附分子-1的影响

Effect of TCM Treatment for Purging Fire, Removing Toxic Substance, Relieving Stagnation and Eliminating Phlegm on Cytoplasmic Free Calcium and Intercellular Adhesion Molecule-1 in Rats with Intracerebral Hemorrhage

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DOI：

中文关键词: 脑出血泻火解毒消瘀除痰〔Ca2+〕i 细胞间黏附分子-1

英文关键词:intracerebral hemorrhage purging fire and removing toxic substance relieving stagnation and eliminating phlegm cytoplasm free calcium intercellular adhesion molecule-1

基金项目:

作者	单位
鲜于开璞	武汉市中西医结合医院中医科武汉430020

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中文摘要:

目的探讨泻火解毒、消瘀除痰法对脑出血(ICH)大鼠钙(〔Ca2+〕i)、细胞间黏附分子-1(ICAM-1)的作用及可能机制。方法将成年SD大鼠随机分为6组:正常对照组、手术对照组、模型对照组、脑清汤(NQT)大、小剂量组、清开灵注射液(QKL)对照组。胶原酶注入右侧尾壳核ICH造模,荧光示踪法检测对ICH大鼠脑细胞〔Ca2+〕i的影响,免疫组织细胞化学技术观察在ICH不同时段对ICAM-1表达的影响。结果NQT大、小剂量组及QKL对照组能显著拮抗脑细胞〔Ca2+〕i升高(P<0.01),显著抑制ICAM-1表达,并使其表达持续时间缩短。结论NQT可阻止钙超载的发生,精密调节细胞因子网络、阻滞细胞因子和炎症介质释放而抗炎,从而保护神经元。

英文摘要:

Objective To study the effects and possible mechanisms of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium (Ca2+i) and intercellular adhesion molecule-1 (ICAM-1) in rats with intracerebral hemorrhage (ICH). Methods Adult SD rats were randomly divided into six groups: the normal group, the operated group, the model group, the Naoqing Decoction (NQD) low- and high-dose groups, and the Qingkailing Injection (QKL) group. ICH model was induced by injecting collagenase into right caudate nucleus, level of Ca2+i was determined by fluorescent tracing assay, and the expression of ICAM-1 was determined using immunohistochemical technique. Results High- and low-dose of NQD and QKL could significantly antagonize the collagenase injection induced increase of Ca2+i (P<0.01), inhibit the expression of ICAM-1 and shorten its sustainable time. Conclusion NQD plays a role in protecting neuron by inhibiting calcium overload, regulating cytokines network, and inhibiting inflammatory reaction through postponing the release of cytokines and inflammatory mediators.

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