内异康复栓对子宫内膜异位症大鼠异位和在位内膜细胞色素C和生存素表达的影响

作者	单位
魏绍斌	四川省中医院成都中医药大学附属医院
曹亚芳	四川省第二中医院
王毅	四川省中医院成都中医药大学附属医院
郭蓉晓	四川省中医院成都中医药大学附属医院
仝崇毅	四川省中医院成都中医药大学附属医院
谭亿民	四川省中医院成都中医药大学附属医院

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中文摘要:

目的探讨内异康复栓直肠给药对子宫内膜异位症(endometriosis,EMT)模型大鼠异位和在位内膜细胞色素C(cytochrome C,cytC)、生存素(Survivin)的不同影响。方法将EMT大鼠随机分为内异康复栓高、低剂量组,丹那唑组,丹莪妇康煎膏组,模型组和空白组,采用免疫组化SP法检测cytC、Survivin的表达。结果(1)内异康复栓高、低剂量组异位内膜cytC(IOD值)表达(分别为6.08±0.35、6.23±0.35)均显著增加,与模型组(5.07±0.70)及丹莪妇康煎膏组(5.98±1.02)比较,差异均有统计学意义(P<0.05,P<0.01);内异康复栓高、低剂量组Survivin(IOD值)表达(分别为5.73±0.93、5.62±0.93)显著降低,与模型组(6.01±1.16)比较,差异均有统计学意义(P<0.05,P<0.01)。(2)内异康复栓高、低剂量组能显著上调在位内膜下降的cytC水平,与模型组比较,差异有统计学意义(P<0.05);内异康复检高、低剂量组在位内膜Survivin的表达,与模型组和空白组比较,差异无统计学意义(P>0.05)。结论内异康复栓可能通过增加异位内膜cytC的表达并降低Survivin的表达,起到诱导凋亡的作用,而对在位内膜可上调cytC表达,对Sur-vivin表达无明显影响。

英文摘要:

Objective To explore the different effects of rectal application of Neiyi Kangfu Suppository(NYKFS)on the expressions of cytochrome C(Cyt C)and Survivin in ectopic and eutopic endometrium in endometriosis(EMT)model rats.Methods EMT rats were randomly divided into 6 groups,the high-and low-dose NYKFS groups,the Danazol Capsule(DN)group,the Dan’e Fukang Soft Extract(DFE)group,the model group and the blank group.The expressions of Cyt C and Survivin were measured using immunohistochemical SP staining method.Results(1)After treatment,the IOD value of Cyt C in ectopic endometrium significantly increased in the high-dose and low-dose NYKFS groups respectively to 6.08±0.35 and 6.23±0.35,which was significantly higher than that in the model group(5.07±0.70)and DFE group(5.98±1.02)respectively(P<0.05,P<0.01);while those of Survivin in ectopic endometrium was significantly decreased to 5.73±0.93 and 5.62±0.93 and was significantly lower than that in the model group(6.01±1.16,P<0.05 or P<0.01).(2)The lowered eutopic endometrial level of Cyt C in EMT rats after treatment was significantly higher in the two NYKFS groups than that in the model group(P<0.05);while that of eutopic endometrial Survivin was insignificantly different between the NYKFS groups and the model group or the blank group(P>0.05).Conclusion NYKFS plays its role in inducing apoptosis by increasing the expression of Cyt C and decreasing the expression of Survivin in ectopic endometrium,but it up-regulates expression of Cyt C,has no obvious effect on Survivin expression in eutopic endometrium.

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