人参皂苷对大鼠肝缺血损伤后糖皮质激素受体的调节作用

作者	单位
封颖璐	解放军401医院干部科第二军医大学附属长海医院中医科
程彬彬	第二军医大学附属长海医院中医科 (山东青岛266071)第二军医大学附属长海医院中医科(上海200433)
凌昌全	第二军医大学附属长海医院中医科 (山东青岛266071)第二军医大学附属长海医院中医科(上海200433)

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中文摘要:

目的研究人参皂苷(GS)是否可以调节大鼠肝缺血后损伤糖皮质激素受体(GR),并就其量效关系做一初步观察,为从GR着手寻找改善肝脏缺血后损伤新方法提供实验依据。方法采用成年雄性SD大鼠建立肝缺血损伤模型;造模前给予100、50、25 mg/kg(大、中、小)不同剂量GS灌胃7天,分别于造模后2、6、12、24 h动态观察肝脏GR的结合活性和GR mRNA的表达水平。结果与正常大鼠比较,模型组及GS各给药组2、6、12 h肝脏GR结合活性和GR mRNA表达明显降低(P<0.01)。与模型组比较,GS中剂量组造模后2、6、12 h肝脏GR结合活性升高和GR mRNA表达增加(均P<0.01),而GS大、小剂量组则无统计学意义。结论GS 50 mg/kg能有效地提高肝缺血损伤大鼠肝脏GR的结合活性和(GR mRNA的表达水平

英文摘要:

Objective To study whether ginsenoside(GS)can regulate the glucocorticoid receptor(GR)in mice with ischemic liver damage,and to preliminarily observe its dose-effect relationship for providing an experi- mental bases in seeking a new way to relieve the damage from view of GR.Methods Adult male SD mouse was used to establish liver ischemia model,and different doses(100,50,and 25 mg/kg)of GS was given via gastric infusion before modeling.The maximal GR binding capacity(Bmax)of liver and the level of GR mRNA expression in liver were dynamically determined at various time points(2 h,6 h,12 h and 24 h)after modeling.Re- sults Compared with the normal control group,GR Bmax and GR mRNA expression in model rats were lower ob- viously(P<0.01).As compared with the control group,GR Bmax and GR mRNA expression in model rats trea- ted with 50 mg/kg GS significantly raised at 2 h,6 h,12 h(P<0.01),while the changes in modeling rats treated with other two doses of GS were of no statistical significance.Conclusion GS in dose of 50 mg/kg can elevate the GR Bmax of liver and the level of GR mRNA expression in liver of rats with ischemic damage.

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