冠心Ⅱ号对大鼠心肌缺血基因差异表达谱的影响

作者	单位
于成瑶	北京世纪坛医院中医科北京100038
高会丽	中国中医科学院西苑医院基础室
刘振	中国中医科学院西苑医院基础室
徐新民	中国协和医科大学北京医院卫生部临床检验中心北京100038
李贻奎	中国中医科学院西苑医院基础室
李连达	中国中医科学院西苑医院基础室

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中文摘要:

目的研究冠心Ⅱ号对大鼠心肌缺血基因差异表达谱的影响。方法6只SD大鼠随机分为3组:假手术组、模型组和冠心Ⅱ号组,每组2只。假手术组及模型组灌胃饮用水,冠心Ⅱ号组按10g/kg灌胃冠心Ⅱ号,灌胃10天后结扎冠状动脉左前降支造成心肌缺血,24h后取心肌缺血交界区组织进行基因芯片检测、聚类分析,并对关键基因差异表达进行RT-PCR验证。结果大鼠心肌缺血后交界区差异表达基因上调基因较多,冠心Ⅱ号干预后,上调和下调基因均减少,突出表现于基因all-trans-13,14-dihydroretinol saturase(AF465614)和similar to expressed sequence AW413625(AA799328)。各组共性基因功能涉及信号转导蛋白活性较多;药物干预基因有一定特异性。结论冠心Ⅱ号对大鼠心肌缺血基因差异表达谱具有一定的特异性改善作用。

英文摘要:

Objective To study the effect of Guanxin No.2(GX2) on gene variant expression profile in rats after myocardial infarction(MI).MethodsSix SD rats were equally randomized into the sham operated group,the model group and the GX2 group,and they received gastric perfusion with water and GX2(10 g/kg) respectively.MI model was established by ligating the left-anterior descending branch of coronary artery after 10 days of perfusion,and rats′ myocardial tissue in the junction zone was assessed 24 h later for gene chip detection with DNA microarray.Then a cluster analysis was conducted,and the different expressions of key genes were verified by real-time reverse transcription-polymerase chain reaction.ResultsThe up-regulating gene expressions in myocardial tissue in the junction zone increased after ischemia.After GX2 intervention,the up-or down-regulating gene expressions,especially the 2 genes,all-trans-13,14-dihydroretinol saturase(AF465614) and similar to expressed sequence AW413625(AA799328) decreased significantly.In the common genes,more genes involving activity of signal transducer presented in the model group and the GX2 group and those in the latter showed a certain specificity.ConclusionGX2 could improve the characteristics of variant gene expression profile in MI rats to a certain extent.

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