针刺肾俞穴对SAMP6小鼠股骨生物力学性能的影响

基金项目:国家自然科学基金重点项目(No．30630074);天津市应用基础及前沿技术研究计划重点项目(No．07JCZDJC08800);教育部高等学校博士学科点专项科研基金(No．320060063006);天津市卫生局中医、中西医结合科研专项资金课题(No．2005034，No．07059);天津市高等学校科技发展基金计划项目(No．20060306)

作者	单位
张雪竹	天津大学化工学院生物工程系
彭应梅	天津中医药大学第一附属医院针灸研究所
于建春	天津中医药大学第一附属医院针灸研究所天津300072
刘存志	天津中医药大学第一附属医院针灸研究所天津300072
韩景献	天津中医药大学第一附属医院针灸研究所天津300072

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中文摘要:

目的探讨针刺肾俞穴治疗骨质疏松的机制。方法采用放免法测定快速老化小鼠P6(senes- cence accelerated mouse prone 6,SAMP6)及其正常对照抗快速老化小鼠R1(senescence accelerated mouse re- sistant 1,SAMR1)血清睾酮(T)、骨钙素(BGP)水平;三点弯曲试验法测定其股骨生物力学性能,观察针刺对SAMP6小鼠股骨力学性能、骨矿含量等的影响。结果与SAMR1[T(20.91±3.41)nmol/L,BGP(6.71±2.07)μg/L]比较,SAMP6小鼠血清T[(11.09±1.48)nmol/,L]水平下降(P<0.01),BGP[(12.29±2.29)μg/L]水平上升(P<0.01);股骨弯曲强度下降,脆性增加。针刺肾俞穴后,上述指标均有不同程度改善,并趋于正常,血清T(15.05±2.63)nmol/L、BGP(8.88±1.85)μg/L,与SAMP6组比较,差异有统计学意义(P<0.05,P<0.01)。结论针刺肾俞穴可有效阻止SAMP6小鼠骨丢失,增强其骨强度。其治疗作用部分是通过促进性激素分泌,改善骨代谢,降低骨转换率,增加骨量等达到的。

英文摘要:

Objective To explore the mechanisms of puncturing Shenshu point in improving osteoporosis. Methods Serum levels of testosterone(T)and osteocalcin(BGP)in senescence accelerated mouse prone 6 (SAMP6,test animals)and senescence accelerated mouse resistant 1(SAMR1,for control)were determined by radioimmunoassay and their femoral biomechanical properties were determined with three-point bending test before and after puncturing to observe the effect of puncturing on the femoral biomechanical properties and bone mineral contents.Results Compared with the SAMRI control group,the serum level of T(20.91±3.41 nmol/L)de- creased(11.09±1.48 nmol/L in SAMP6 mouse),BGP(6.7±2.07μg/L)increased(12.29±2.29μg/L in SAMP6 mouse),femoral bending strength lowered and fragility increased.These changes were all improved to some extent or normalized,serum T level 15.05±2.63 nmol/L and BGP 8.88±1.85μg/L after needling at Shen- shu point showed significant difference when compared with those in SAMP6.Conclusion Puncturing Shenshu point could effectively prevent the bone loss in SAMP6 mice,increase their bone strength,the therapeutic effect is partly by way of promoting the secretion of sex hormone,improving bone metabolism,suppressing bone transforma- tion rate and increasing bone minerals.

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