淫羊藿总黄酮延缓秀丽隐杆线虫衰老的实验研究

作者	单位
蔡外娇	复旦大学附属华山医院中西结合研究所上海200040
张新民	复旦大学附属华山医院中西结合研究所上海200040
黄建华	复旦大学附属华山医院中西结合研究所上海200040
沈自尹	复旦大学附属华山医院中西结合研究所上海200040

摘要点击次数: 1604

全文下载次数: 3

中文摘要:

目的以热量限制(caloric restriction,CR)为阳性对照,评价淫羊藿总黄酮(epimedium fla- voniods,EF)延缓模式生物秀丽隐杆线虫衰老的效果并为进一步的机制研究奠定基础。方法(1)寿命分析实验中,动物分组为空白组,CR组,EF高、低剂量组,观察平均寿命、最大寿命、年龄依赖死亡率(age-depend- ent mortality);(2)生殖能力测试中,动物分组为空白组与EF高剂量组,通过计数总子代数目测试生殖能力;(3)压力应激实验中,动物分组为空白组与EF高剂量组,通过观察35℃急性热应激下(acute heat-stress)的生存时间测试压力应激能力。结果(1)与空白组比较,EF低剂量组平均寿命延长(P<0.05),高剂量组平均寿命及最大寿命均显著延长(P<0.01)、年龄依赖死亡率降低;与CR组比较,EF高剂量组平均寿命和最大寿命均显著延长(P<0.01)。(2)与空白组比较,EF高剂量组子代数目差异无统计学意义(P>0.05)。(3)与空白组比较,EF高剂量组在35℃急性热应激下的平均生存时间显著延长(P<0.01)。结论EF延缓线虫衰老同时不损害生殖能力,并能显著提高线虫在急性热应激下的应激能力。

英文摘要:

Objective To investigate effect of Epimedium flavonoids(EF),positively controlled by caloric restriction(CR)method,in retarding aging of the model organism C.elegans,in order to establish a basis for studying its action mechanism.Methods Experiment for life-time analysis was conducted on animals grouped into the blank group,the CR group,and the high and low dose EF groups to observe their mean lifespan,maximum li- fespan and age-dependent mortality.And the reproductive capacity test and acute heat-stress analysis were carried out in the blank group and the high dose EF group to observe the subalgebra and the mean survival time under acute heat-stress at 35℃.Results As compared with the blank group,the mean lifespan in the two EF group and the maximum lifespan in the high dose EF group were higher,and the age-dependent mortality in the high dose EF group was lower significantly(P<0.05 or P<0.01);as compared with the CR group,the mean lifespan and maximum lifespan in the high dose EF group were higher(P<0.01);but no significant difference of the subalge- bra between the blank group and the high dose EF group was shown(P>0.05).Compared with the blank group, the mean lifespan in the high dose EF group was significantly prolonged under acute heat-stress at 35℃(P<0.01).Conclusion EF can retard the aging of C.elegans without damage on the reproductive capacity,and sig- nificantly improve its capacity against acute heat-stress.

关闭