加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠病理模型的影响

作者	单位
孙海芸	北京中医药大学东直门医院中药药理学实验室北京100700
任映	北京中医药大学东直门医院中药药理学实验室北京100700
尹军祥	北京中医药大学东直门医院中药药理学实验室北京100700
李本志	北京中医药大学东直门医院中药药理学实验室北京100700
叶永安	北京中医药大学东直门医院中药药理学实验室北京100700
杨金铎	北京中医药大学东直门医院中药药理学实验室北京100700
崔界蜂	北京中医药大学东直门医院中药药理学实验室北京100700
谭玮	北京中医药大学东直门医院中药药理学实验室北京100700
宋崇顺	北京中医药大学东直门医院中药药理学实验室北京100700

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中文摘要:

目的对加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠的病理和病变程度进行定量分析。方法采用人血清白蛋白(8 g/L)对大鼠进行皮下免疫,在大鼠产生抗体后分组[将造模大鼠随机分为6组,即加味鳖甲煎丸大(13g/kg)、中(6.5g/kg)、小(3.25g/kg)剂量组(简称为鳖甲大、中、小剂量组),模型组,阳性药秋水仙碱(1.0 mg/kg)对照组(简称秋水仙组),阳性药肝脾康(22.23 mg/kg)对照组(简称为肝脾康组)]给药,给药后40 min再由尾静脉注射人血清白蛋白,即可得到肝纤维化实验动物模型,实验结束后处死大鼠,病理切片,HE和Masson染色,对肝组织病理变化、胶原纤维含量进行分析。结果与模型组比较,鳖甲大、中、小剂量组能显著改善肝纤维化大鼠病变程度(总分降低率分别为62.50%、40.75%、8.33%);胶原纤维含量亦明显减少(P<0.05,P<0.01)。结论加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠病变程度有明显防治作用。

英文摘要:

Objective To quantitatively analyze the effect of modified Biejiajian Pill(BJJP)on the pathologi- cal change and degree of albumin induced immune hepatic fibrosis in rats.Methods Rats were immunized by multiple subcutaneous injections of human serum albumin(8 g/L),and were medicated in groups respectively after antibody producing,BJJP high-dose(13 g/kg)group,medium-dose(6.5 g/kg)group,low-dose(3.25 g/kg) group,the model group,colehicines(1.0 mg/kg)group,and Ganpikang(22.23 mg/kg)group.Then,caudal vein injection of albumin was given 40 min after medication to induce liver fibrosis.Animals were sacrificed finally to observe the pathological change,and the distribution and content of collagen and plastin were determined quanti- tatively with HE and Masson stain.Results BJJP high-,medium-,and low-dose groups could obviously improve the pathological change of the hepatic fibrosis rats(decreasing rate of the total score was 62.50%,40.75%,and 8.33%,respectively),and the content of collagen reduced markedly(P<0.05,P<0.01).Conclusion BJJP can effectively prevent and reduce the pathological change of albumin induced immune hepatic fibrosis in rats.

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