滋阴泻火中药对雌性青春期大鼠生长板ERα、IGF-1R及EGFR基因表达和蛋白合成的调节作用

Regulative Effects of Chinese Herbs for Nourishing Yin and Removing Fire on Gene Expressions of Estrogen Receptor α,Insulin-like Growth Factor-1 Receptor,Epithelial Growth Factor Receptor and Protein Synthesis in Epiphyseal Growth Plate of Female Puberta

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中文关键词: 滋阴降火中药生长板雌激素受体α 胰岛素样生长因子-1受体表皮细胞生长因子受体

英文关键词:Chinese herbs for nourishing yin and removing fire epiphyseal growth plate estrogen receptor α insulin-like growth factor-1 receptor epithelial growth factor receptor

基金项目:国家自然科学基金资助项目(No.30371820)

作者	单位
芦军萍	复旦大学附属儿科医院
蔡德培	复旦大学附属儿科医院

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中文摘要:

目的观察滋阴泻火中药对雌性青春期大鼠生长板雌激素受体α(ERα)、胰岛素样生长因子-1受体(IGF-1R)及表皮细胞生长因子受体(EGFR)基因表达和蛋白合成的调节作用,探讨滋阴泻火中药改善骨骼发育的作用环节和作用机制。方法将青春期大鼠分为对照组和中药干预组,采用免疫组织化学和实时定量-聚合酶链反应(Realtime-PCR)对干骺端生长板ERα、IGF-1R和EGFR的基因表达和蛋白合成情况进行检测。结果给予滋阴泻火中药干预后,青春期大鼠干骺端生长板ERα和IGF-1R的基因表达明显下调,蛋白合成显著减少;EGFR的基因表达和蛋白合成无明显改变。结论滋阴泻火中药可以通过调节生长板ERα和IGF-1R的表达,对骨骼的发育和成熟发挥调节作用。

英文摘要:

Objective To investigate the effect of Chinese herbs for nourishing yin and removing fire(NYRF)on gene expressions of estrogen receptor α(ERα),insulin-like growth factor-1 receptor(IGF-1R)and epithelial growth factor receptor(EGFR)in the epiphyseal growth plate of the female pubertal rats.Methods The rats were randomly divided into the control group and the intervened group.Immunohistochemistry and realtime-PCR methods were used to measure the gene expression of ERα,IGF-1R and EGFR and their protein synthesis in epiphyseal growth plate.Results After being intervened with NYRF,the gene expressions of ERα and IGF-1R were down-regulated and their protein synthesis markedly reduced,while those of EGFR were unchanged.Conclusion NYRF can modulate the development and maturation of bone by regulating the expressions of ERα and IGF-1R in the epiphyseal growth plate.

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