荣肝合剂对ConA诱导慢性免疫性肝损伤小鼠细胞凋亡功能的影响

作者	单位
王凤云	中国中医科学院西苑医院实验中心
唐旭东	西苑医院消化科
许勇钢	中国中医科学院西苑医院实验中心
杨晓红	中国中医科学院西苑医院实验中心
张引强	西苑医院消化科

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中文摘要:

目的观察刀豆蛋白(ConA)诱导转基因小鼠慢性免疫性肝损伤肝组织淋巴细胞中Fasl和Bcl-2蛋白表达情况,探讨荣肝合剂对细胞凋亡的影响。方法选用转基因小鼠74只,随机分为模型组、正常组、联苯双酯(DDB)组、茵陈组、茵陈蒿汤组、荣肝合剂组,分别灌胃给药4周。末次药后24 h,取肝组织光镜下观察其病理变化,TUNEL法检测细胞凋亡数,流式细胞仪检测肝组织T淋巴细胞中Fasl和Bcl-2蛋白表达。结果病理组织学变化:与模型组比较,荣肝合剂组及茵陈蒿汤组肝组织纤维增生及细胞坏死较轻(P<0.05);凋亡指数及相关基因蛋白的表达:与正常组比较,模型组的凋亡指数及凋亡基因Fasl蛋白表达显著增高(P<0.05),而凋亡抑制基因Bcl-2蛋白表达无显著变化(P>0.05);与模型组比较,荣肝合剂组及茵陈蒿汤组凋亡指数减少(P<0.01),并且两组的Fasl表达水平显著下降(P<0.05),Bcl-2表达水平明显升高(P<0.01);与茵陈蒿汤组比较,荣肝合剂组在凋亡指数及Fasl和Bcl-2蛋白表达方面差异无统计学意义(P>0.05),而与茵陈组和联苯双酯组比较,差异有统计学意义(P<0.05或P<0.01)。结论中药复方(荣肝合剂和茵陈蒿汤)不仅可减轻肝组织的病理损害,还可能通过对Fasl和Bcl-2表达的调节,减少慢性免疫性肝损伤小鼠细胞凋亡。

英文摘要:

Objective To explore the effects of Ronggan Mixture (RGM) on cell apoptosis by observing the expressions of apoptosis-related genes (Fasl and Bcl-2) in transgenic mice with chronic liver immune injury in- duced by concanavalin A (ConA).Methods Seventy-four transgenic mice were divided into 6 groups,the model group,the normal group,and the treated groups treated respectively with biphenyldicarboxylate (DDB),oriental wormwood (OWW),Yinchenhao Decoction (YCHD) and RGM.Pathologic changes of liver tissue were observed by light microscopy,number of apoptotic cells were determined by TUNEL method,and expressions of apoptosis-re- lated genes,Fasl and Bcl-2,in hepatic T lymphocyte were detected by flow cytometer.Results Evident pathologi- cal changes of liver appeared in the model mice,showed severely destroyed structure of hepatic lobules.As com- pared with the model group,the changes of liver fibrosis and cell necrosis were much lessened in the RGM group and the YCHD group (P<0.05).The protein expression of apoptotic gene Fasl and the apoptotic index in the model group were higher than those in the normal group (P<0.05),but that of the apoptotic inhibiting gene,Bcl- 2,in model mice was similar to that in normal mice.As compared with the model group,apoptosis index decreased (P<0.01),levels of Fasl expression was lower and Bcl-2 expression was higher in the RGM group and the YCHD group (P<0.05,P<0.01),and the effect of the two was similar,but significantly superior to that of OWW and DDB (P<0.05 or P<0.01).Conclusion The Chinese compound,RGM and YCHD can not only relieve the he- patic pathological injury,but also reduce the cell apoptosis in chronic liver immune injury mice through regulating the expressions of Fasl and Bcl-2.

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