甘草次酸对白血病细胞侵袭力及明胶酶活性的影响

作者	单位
张金玲	华中科技大学同济医学院附属协和医院血液病研究所
刘新月	华中科技大学同济医学院附属协和医院血液病研究所
邹萍	华中科技大学同济医学院附属协和医院血液病研究所

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中文摘要:

目的研究甘草次酸(glycyrrhetinic acid,GA)对白血病细胞侵袭能力及明胶酶表达的影响。方法对体外培养的白血病细胞系K562、HL-60,用MTT法检测不同浓度GA对细胞增殖的影响;采用Tran-swell小室基质胶侵袭实验观察GA对K562、HL-60细胞株侵袭能力的影响;明胶酶谱法检测GA对细胞明胶酶活性的影响。结果GA对K562、HL-60细胞有明显的增殖抑制作用;能够以浓度依赖性的方式抑制K562、HL-60细胞的侵袭能力;GA能够显著下调K562、HL-60细胞明胶酶的A、B活性。结论GA能够抑制白血病细胞K562、HL-60明胶酶活性,从而抑制K562、HL-60细胞的侵袭能力,为白血病细胞髓外浸润的预防提供实验依据。

英文摘要:

Objective To study the effect of glycyrrhetinic acid(GA)on the invasive capacity of leukemic cells and the activity of intracellular gelatinase.Methods The effect of GA,in different concentrations,on the proliferation of cultured K562 and HL-60 leukemic cells in vitro was determined by MTT assay;that on cell invasive capacity was tested by Transwell cubicle matrigel invasion assay;and that on the activity of gelatinase in cells was detected by gelatin zymography.Results GA showed significant inhibitory effect on the proliferation of K562 and HL-60 leukemic cells;it inhibited the invasive capacity of cells in concentration-dependent manner;and significantly down-regulated the activity of gelatinase A and B in cells.Conclusions GA can inhibit invasive capacity of K562 and HL-60 leukemia cells by way of suppressing the activity of gelatinase A and B.This study provides an experimental evidence for preventing extra-medullary infiltration of leukemic cells.

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