苦参碱对白血病细胞株Jurkat侵袭转移的影响

作者	单位
张伟	重庆医科大学附属儿童医院
戴碧涛	重庆医科大学附属儿童医院
徐酉华	重庆医科大学附属儿童医院
于洁	重庆医科大学附属儿童医院
王世一	重庆医科大学附属儿童医院
宪莹	重庆医科大学附属儿童医院
刘筱梅	重庆医科大学附属儿童医院

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中文摘要:

目的研究不同浓度苦参碱对人急性淋巴细胞白血病细胞株Jurkat侵袭转移的抑制作用。方法Jurkat细胞体外培养,经0g/L、0.1g/L、0.15g/L、0.2g/L苦参碱分别处理细胞,细胞黏附实验检测细胞黏附能力,细胞迁移实验检测细胞运动能力,肿瘤细胞体外侵袭实验检测细胞侵袭能力,RT-PCR检测细胞基质金属蛋白酶(MMP-2和MMP-9)mRNA的表达,并采用直线相关分析Jurkat细胞MMP-9 mRNA的表达与细胞侵袭力之间的线性相关程度。计量资料的多组比较采用方差分析。结果与对照组比较,0.15g/L、0.2g/L苦参碱能有效抑制Jurkat细胞与纤维粘连蛋白(FN)的黏附能力(P<0.05),0.1g/L、0.15g/L、0.2g/L苦参碱能显著降低Jurkat细胞的运动能力和侵袭能力(P<0.01)。MMP-2在Jurkat细胞中无明显表达,MMP-9在Jurkat细胞中高表达,0.1g/L、0.15g/L、0.2g/L苦参碱能明显下调Jurkat细胞MMP-9 mRNA的表达(P<0.01),Jurkat细胞MMP-9 mRNA的表达与细胞侵袭力呈正相关(r=0.940,P<0.01)。结论苦参碱能全面抑制Jurkat细胞的黏附能力、运动能力和侵袭能力,其作用机制可能与下调MMP-9mRNA的表达有关,是一种较好的抗白血病细胞侵袭转移的药物。

英文摘要:

Objective To study the inhibitory effects of matrine,in different concentrations,on invasion and metastasis of human acute lymphocytic leukemia cell line Jurkat.Methods In vitro cultured Jurkat cells were treated by matrine in concentration of 0 g/L,0.1 g/L,0.15 g/L and 0.2 g/L,respectively.Then cell adhesion assay,cell migration assay and matrigel invasion assay were used respectively to observe the effects of matrine on adhesion,migration and invasive capacity of Jurkat cells.Meantime,RT-PCR was performed to detect the matrix metalloproteinase(MMP)-2 and MMP-9 mRNA expression levels.Comparison of measurement data among groups was analyzed by variance analysis.Results As compared with the control group,the adhesion of Jurkat to fibronectin(FN)was significantly inhibited by 0.15 g/L and 0.2 g/L of matrine(P<0.05);the cell migration and invasive capacity were significantly lowered by 0.1 g/L,0.15 g/L and 0.2 g/L matrine(P<0.01).High mRNA expression of MMP-9 presented but that of MMP-2 was expressed insignificantly in Jurkat cells,matrine at 0.1 g/L,0.15 g/L and 0.2 g/L showed obvious effect in down-regulating MMP-9 mRNA expression(P<0.01).Besides,MMP-9 mRNA expression was found to be positively correlated with the invasive capacity of Jurkat cells(r=0.940,P<0.01).Conclusions Matrine is a good drug for antagonizing the invasion and metastasis of leukemia cells,it may roundly inhibit the adhesion,migration and invasive capacity of Jurkat cells,the mechanism might be related with the down-regulation of MMP-9 mRNA expression.

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