黄芪牛蒡子不同配伍对STZ大鼠肾组织内活性氧产物含量和核转录因子-κB表达的影响

Effect of Astragalus and Arctium in Different Combinations on Reactive Oxygen Species Content and Nuclear Transcription Factor κB Expression in Renal Tissue of Streptozotocin Rats

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中文关键词: 糖尿病肾病活性氧产物核转录因子-κB 黄芪牛蒡子正交设计

英文关键词:diabetic nephropathy reactive oxygen species nuclear transcription factor-kappa B Astragalus Arctium orthogonal design

基金项目:上海市科学技术发展基金资助项目(No.99047)

作者	单位
王巍巍	中国人民解放军第四五五医院肾脏科
陈以平	上海市龙华医院

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中文摘要:

目的探讨糖尿病时肾组织内活性氧产物(ROS)含量和核转录因子-κB(NF-κB)的表达及黄芪牛蒡子配伍对其的影响。方法建立STZ大鼠模型,将黄芪牛蒡子各分为高、中、低剂量,按照正交设计进行不同剂量的配伍组合,灌胃4周或8周。实验第4及第8周时,流式细胞术检测大鼠肾组织内ROS含量,蛋白免疫印迹方法检测NF-κBp65活性表达。结果实验第4周及第8周时,与正常大鼠相比,糖尿病模型大鼠肾组织内ROS含量(%,模型组、正常组分别为第4周:36.55±7.46、6.21±1.83;第8周:31.91±5.83、6.95±1.41)及NF-κBp65的蛋白表达(灰度值,模型组、正常组分别为第4周:165.00±3.14、129.00±1.58;第8周:214.00±5.11、148.00±2.32)明显升高;黄芪牛蒡子配伍可减少其表达,并以黄芪中高剂量为主的配伍组合作用较好〔第4周时,黄芪高、中、低剂量配伍组ROS含量(%)分别为11.43±2.42、18.37±7.58、22.10±4.71;第8周时为12.55±4.40、19.15±6.64、23.48±3.13;第8周时NF-κBp65表达量(灰度值)黄芪高、中、低剂量配伍组分别为185.00±6.99、183.00±3.89、194.00±4.98〕。结论黄芪牛蒡子配伍可能通过抑制ROS-NF-κB信号通路的活化,减轻糖尿病肾脏病变。

英文摘要:

Objective To investigate the reactive oxygen species(ROS)content and nuclear transcription factor-kappa B expression(NF-κB)in renal tissue of diabetic rats and the effect of Astragalus and Arctium in combination on them.Methods Streptozotocin(STZ)-induced diabetic model rats were established.Astragalus and Arctium in different dosages(low,moderate,high)were combined after orthogonal design and administered respectively to the model rats via gastrogavage for 4 or 8 weeks.Content of ROS in the renal tissue was detected by flow cytometry and expression of functional NF-κB p65 was assessed by Western blot at the 4th and 8th weekends(4th week and 8th week).Results ROS content of renal tissue in the model rats was 36.55±7.46% at the 4th week and 31.91±5.83% at the 8th week,NF-κB p65 expression was 165.00±3.14 at the 4th week and 214.00±5.11 at the 8th week,all higher than those in normal rats(6.21±1.83% and 129.00±1.58 at the 4th week,6.95±1.41% and 148.00±2.32 at the 8th week)respectively.The combined use of Astragalus and Arctium showed decreasing effects on both indexes significantly,and the decreasing effects of the combination with moderate and high dose Astragalus were better than those with low dose,with the details as follows:those of ROS at the 4th week were 11.43±2.42%,18.37±7.58% and 22.10±4.71% for high,moderate and low dose Astragalus combination respectively(same hereinafter),at the 8th week 12.55±4.40%,19.15±6.64% and 23.48±3.13%;and for NF-κB p65 expression at the 8th week,185.00±6.99,183.00±3.89 and 194.00±4.98 respectively.Conclusion Combined use of Astragalus and Arctium may ameliorate the condition of diabetic nephropathy by inhibiting the activation of the ROS-NF-κB signal passage.

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