川芎嗪和黄芪在子宫内膜异位症发生发展中对RANTES及受体的调节作用

Regulatory Effect of Ligustrazine and Radix Astragalus on RANTES and Receptor CCR5 Expression in the Genesis and Development Process of Endometriosis

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中文关键词: 子宫内膜异位症间质细胞细胞培养 RANTES CCR5 川芎嗪黄芪

英文关键词:endometriosis stromal cell cell culture RANTES CCR5 ligustrazine Radix Astragalus

基金项目:上海市卫生局资助课题(No.034105)

作者	单位
闵治红	复旦大学附属妇产科医院
张冀宁	复旦大学附属妇产科医院
华克勤	同济大学附属上海市第一妇婴保健院

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中文摘要:

目的探讨益气活血化瘀中药川芎嗪和黄芪对子宫内膜异位症患者子宫内膜间质细胞趋化因子(regulated on activation,normal Tcell expressed and secreted,RANTES)和受体CCR5表达水平的调节作用。方法选择卵巢内膜样囊肿患者10例为试验组,其他子宫良性病变患者10例为对照组,分离和纯化在位和异位子宫内膜间质细胞,加入不同干扰因素(黄芪注射液、川芎嗪注射液、黄芪联合川芎嗪注射液、丹那唑),用ELISA方法和逆转录多聚酶链反应(RT-PCR)测RANTES和CCR5表达水平。结果(1)异位子宫内膜间质细胞表达RANTES水平与在位的表达水平比较差异无统计学意义(P>0.05);异位子宫内膜间质细胞表达RANTES水平(ng/L)阳性对照组(13.602±3.358)较阴性对照组(0.027±0.016)明显增高(P<0.05),在位子宫内膜间质细胞表达RANTES水平阳性对照组(12.850±7.997)较阴性对照组(0.027±0.016)明显增高(P<0.05)。加入不同中药干预因素后,异位子宫内膜间质细胞表达RANTES水平较阳性对照组明显下降(P<0.05)。(2)异位子宫内膜间质细胞表达CCR5水平(0.759±0.039)较在位子宫内膜(0.249±0.026)明显增高(P<0.05)。异位子宫内膜间质细胞表达CCR5水平阳性对照组(0.759±0.039)较阴性对照组(0.478±0.094)明显增高(P<0.01),在位子宫内膜间质细胞表达CCR5水平阳性对照组(0.249±0.026)较阴性对照组(0.131±0.021)明显增高(P<0.01)。加入不同中药干预因素后,CCR5表达水平较阳性对照组均明显下降(P<0.01)。结论子宫内膜异位症患者异位子宫内膜较在位子宫内膜间质细胞表达CCR5水平高。川芎嗪和黄芪对子宫内膜异位症患者RANTES及其受体CCR5的自分泌作用具有明显降调节作用。

英文摘要:

Objective To investigate the regulatory effect of ligustrazine and Radix Astragalus(Chinese drugs for supplementing qi and activating blood circulation)on uterine mucosa stromal cyto-chemotactic factor RANTES and receptor CCR5 expression.Methods The eutopic and ectopic endometrial stromal cells(EMSC)isolated and purified from 10 patients with ovarian endometrial cyst were selected as the experimental group,while those from 10 patients with benign lesion were selected as the control group.After they were intervened by different factors,including astragalus injection(AI),ligustrazine injection(LI),AI+LI,and Danazol,the expression levels of RANTES and CCR5 in the cells were detected by ELISA and RT-PCR.Results RANTES expressions in eutopic and ectopic EMSC were different insignificantly(P>0.05).The ectopic EMSC RANTES expression was 13.602±3.358 ng/L and eutopic EMSC RANTES was 12.850±7.997 ng/L in the positive control group,which was significantly higher than that in the negative control group(both being 0.027±0.016 ng/L,P<0.05),and the ectopic EMSC expressions after intervened with Chinese drugs were significantly lower than that in the positive control group(P<0.05).CCR5 expression in ectopic EMSC was 0.759±0.039 ng/L,which was higher than that in eutopic EMSC(0.249±0.026 ng/L,P<0.05).Ectopic EMSC CCR5 expression was higher in the positive control group than that in the negative control group(0.759±0.039 ng/L vs 0.478±0.094 ng/L,P<0.05).Similar situation also was shown between the positive and negative control groups in terms of eutopic EMSC CCR5 expression(0.249±0.026 ng/L vs 0.131±0.021 ng/L,P<0.01),and the expression was significantly lower in all the Chinese drugs treated EMSC groups as compared with that in the positive control group(P<0.01).Conclusion CCR5 expression was higher in ectopic EMSC than that in eutopic EMSC.Ligustrazine and Radix Astragalus could down-regulate the auto-secretion of RANTES and CCR5 in patients with endometriosis.

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