芎芍胶囊对兔动脉粥样硬化模型脂质代谢及血小板聚集的影响

作者	单位
李立志	中国中医科学院西苑医院心血管病中心
刘剑刚	中国中医科学院西苑医院心血管病中心
马鲁波	中国中医科学院西苑医院心血管病中心
鹿小燕	卫生部中日友好医院心血管病中心
徐浩	卫生部中日友好医院心血管病中心
徐凤芹	中国中医科学院西苑医院心血管病中心
史大卓	中国中医科学院西苑医院心血管病中心
陈可冀	中国中医科学院西苑医院心血管病中心卫生部中日友好医院心血管病中心

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中文摘要:

目的观察芎芍胶囊对兔动脉粥样硬化模型脂质代谢及血小板聚集的影响并探讨其可能机制。方法用4F·Forgarty导管剥脱损伤腹主动脉内皮后继喂高胆固醇饲料的方法,复制节段性兔动脉粥样硬化模型。70只动物术后随机分为7组,即模型3天、2周、6周组(A、B、C组),单纯内皮损伤组(D组),普罗布考组(E组),芎芍胶囊小剂量组(F组),芎芍胶囊大剂量组(G组),另10只为假手术组(N组)。除D组和N组外,其余各组均在高胆固醇饲料基础上分别喂以不同种类及剂量的药物。在各观察时点,动物处死前心脏抽血测定TC、TG、HDL-C、LDL-C等指标;术后3天及实验结束时心脏取血,测定5 min内血小板最大聚集率(mPAGR)。结果(1)对AS兔血脂水平的影响:D组血脂水平与N组比较无明显变化(P>0.05);动物喂食高脂饲料3天后TC、LDL-C即明显升高,A、B、C组与N组比较差异均有统计学意义(P<0.05,P<0.01);实验结束时,与C组比较,G、E组TC、LDL-C均明显降低,G、E、F各组均能降低LDL-C/HDL-C比值,尤以G组明显(P<0.01);G组尚有升高HDL-C的趋势,E组变化不明显。G组可明显降低致动脉粥样硬化指数(AI,TC/HDL-C),与C、E组比较,差异有统计学意义(P<0.05)。(2)对血小板聚集的影响:内皮损伤后3天A、B、C组及D组mPAGR均明显升高,与N组比较差异均有统计学意义(P<0.01);G、E、F各组均明显降低术后3天mPAGR,与C组比较,G组差异有统计学意义(P<0.05)。G、E、F各组均可降低术后6周mPAGR,与C组比较有统计学意义(P<0.05,P<0.01),以G组作用更为显著。结论芎芍胶囊可降低兔动脉粥样硬化模型TC、LDL-C水平及动脉粥样硬化指数AI,还可抑制该模型的早期及术后6周血小板集聚,这些可能是其抗AS的机制之一。

英文摘要:

Objective To study the effect and possible mechanism of Xiongshao Capsule (XSC) on lipid me- tabolism and platelet aggregation in experimental atherosclerotic (AS) rabbits.Methods Fractional AS rabbits model was established by denuding endothelium of abdominal aorta with 4F·Fogarty catheter,followed by high cholesterol feeding.Seventy model rabbits were equally randomized into 7 groups,namely,the 3 model groups (Group A,B and C) of different observation time (3 days,2 weeks and 6 weeks respectively after operation) ;the single endothelium injury group (Group D,adopted denuding but fed with common diet) ;the 3 treatment groups treated respectively with probucol (Group E),low-dose and high-dose XSC (Group F and G) for 6 weeks.Be- sides,a control group (Group N) consisting of 10 rabbits underwent sham operation with normal feeding was set up.Animals were killed at different corresponding time points,heart blood was collected before killing for measure- ments of blood lipid indexes,including total cholesterol (TC),total triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C).The 5 min maximum platelet aggregation rate (mPAGR) was measured with heart blood on the 3rd day after operation and at the terminal of experiment. Results (1) Indexes of blood lipids were unchanged in Group D and N (P>0.05);TC and LDL-C in model rabbits began to rise significantly after being fed with high fat forage for 3 days;lipid indexes in Group A,B and C were significantly different to those in Group N,respectively (P<0.05,P<0.01);at the end of experiment TC and LDL-C were lower in Group G and E when compared with Group C;the ratio of LDL-C/HDT-C decreased in Group G,E and F,especially in Group G(P<0.01);a bettering trend of HDL-C presented in Group G,but not in Group E;and reduction of atherosclerosis index (AI,calculated by TC/I-IDL-C) was found in Group G (P<0.01 ),significant difference was shown as compared with that in Group C and E (P<0.05).(2) The 3rd day mPAGR raised in Group A,B,C and D,showing significant difference as compared with that in Group N (P<0.01),whereas the index in Group E,F and especially in G was lower significantly than that in Group C (P<0.05).mPAGR in the 3 treatment groups at the terminal of experiment was lower than that in Group C (P<0.05, P<0.01),and the most effective lowering was shown in Group G.Conclusion XSC could reduce the levels of TC,LDL-C and AI in AS model rabbits,also inhibit platelet aggregation at early stage and 6 weeks after endotheli- um injury,which might be one of the possible acting mechanisms of XSC in preventing AS.

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