加味麻黄附子细辛汤提取物对缺血再灌注诱发新西兰兔房室传导阻滞的影响

目的观察加味麻黄附子细辛汤提取物对缺血再灌注诱发新西兰兔房室传导阻滞模型的影响。方法将新西兰兔随机分6组,即:结扎右冠状动脉15 min(对照及实验A组)、60 min(对照及实验B组)、120 min(对照及实验C组),每组10只。实验组给予加味麻黄附子汤提取物、对照组给予生理盐水,均空腹灌胃,每次2 mL/kg,2次/天,连续灌胃3天。末次灌胃20 min后,20%乌拉坦1.2 g/kg耳缘静脉注射麻醉,结扎右冠状动脉制备缺血再灌注诱发房室传导阻滞动物模型,记录各组不同时段的Ⅱ导心电图和希氏束电图。观察P-R间期及A-H间期。结果缺血实验中,实验组动物P-R间期和A-H间期,于结扎5 min后较模型动物缩短,差异有统计学意义(P<0.05)。缺血再灌注实验中,复灌后实验组与对照组相比,P-R间期和A-H间期缩短差异有统计学意义(P<0.05);长时间(120 min)缺血组,于复灌60 min后对照动物P-R间期、A-H间期再次延长,实验组动物未出现再次延长现象,差异有统计学意义(P<0.05)。结论加味麻黄附子细辛汤可改善缺血、缺血再灌注引起的房室传导功能。

英文摘要:

Objective To study the effects of modified Mahuang Fuzi Xixin Decoction extract (MFXDE) on ischemia/reperfusion induced atrioventricular (A-V) block in rabbits model.Methods New Zealand rabbits were randomly divided into six groups,three test groups (Group TA,TB,TC) and three control groups (Group CA, CB,CC),10 in each.MFXDE 2 mL/kg was given twice a day by gastrogavage to the test groups,while to the control groups,equal volume of normal saline was given instead,for 3 successive days.Twenty minutes after the last gastrogavage,right coronary artery ligation was performed in rabbits under anesthesia with 1.2 g/kg of 20% u- rethane via ear marginal vein injection,and lasted for 15 min (Group TA and CA),60 rain (Group TB and CB) , and 120 min (Group TC and CC),respectively.ECG lead-H and His Bundle ECG were recorded at different time points to observe P-R interval and A-H interval.Results P-R interval and A-H interval in the test groups were shorter than in the control groups significantly (all P<0.05) at the time point of 5 min after ischemia;and at the reperfusion stage,a re-extending phenomenon of P-R and A-H could be found in the CC group after 60 min reper- fusion (P<0.05),but it didn’t occure in all the other test groups.Conclusion MFXDE could improve the is- chemia/reperfusion induced A-V conductive function.

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