癃必消胶囊对体外培养的人前列腺增生间质细胞作用机制的实验研究

作者	单位
贾金铭	中国中医科学院广安门医院男科
马卫国	中国中医科学院广安门医院男科
焦拥政	中国中医科学院广安门医院男科
王家辉	中国中医科学院广安门医院男科
罗少波	中国中医科学院广安门医院男科
张琚	南开大学生物活性材料研究所
张智松	南开大学生物活性材料研究所

摘要点击次数: 1261

全文下载次数: 3

中文摘要:

目的研究中药癃必消胶囊对体外培养的人前列腺增生间质细胞的作用机制。方法将雄性日本大耳白兔9只随机分为中药高剂量、低剂量组和对照组,每组3只,中药高、低剂量组分别予癃必消胶囊药粉〔2.0g/(kg.d),1.0g/(kg.d)〕灌胃,对照组予等体积生理盐水灌胃。每天灌胃2次,间隔12h,连续3天。采用最后一次灌药3h后的血清加入细胞培养液。将含癃必消胶囊药物的兔血清加入到体外培养的人前列腺增生间质细胞中,采用TUNEL、ELISA、免疫细胞化学等相关技术检测间质细胞的增殖及凋亡水平。采用实时定量RT-PCR等技术,检测转化生长因子β1(TGF-β1)和Smad7在前列腺增生间质细胞中的表达。结果各组细胞增殖均表现出用药时间依赖性,与对照组比较,各用药组细胞增殖水平显著降低,差异有统计学意义(P<0.01),与低剂量组比较,高剂量组增殖降低,差异有统计学意义(P<0.01)。各组培养48h的前列腺间质细胞,在形态上并未出现明显的不良生长状况,各组间差异均无统计学意义。对照组和低剂量组未见凋亡现象,只有高剂量组出现少量凋亡细胞。低、高剂量组TGF-β1、Smad7表达水平均低于对照组,差异均有统计学意义(均P<0.01)。低剂量组与高剂量组比较,差异无统计学意义(P>0.05)。结论癃必消胶囊能降低基因TGF-β1和Smad7的表达,抑制前列腺间质细胞增殖,而对其凋亡无明显的影响。

英文摘要:

Objective To explore the mechanism of action of Longbixiao Capsule (LBXC,a Chinese herbal preparation) on human prostatic stromal cell cultured in vitro. Methods Nine Japan rabbits were assigned to 3 groups.The high,low dose group was given LBXC 〔2.0 g/(kg·d),1.0 g/(kg·d)〕 by gastro gavage respectively,while equal volume of normal saline was given by gastrog avage 60 rats in the control group,all twice a day with an infeval of 12 h,for 3 successive days. The serum collected at 3 h after the last gastro gavage was added into cell culture fluid.Rabbit′s serum containing LBXC was incubated with the cultured stromal cells,and the levels of cell proliferation and apoptosis were determined using relative techniques as TUNEL,ELISA,and immunocytochemistry. Besides,Real-time RT-PCR was applied to detect the mRNA expressions of TGF-β1 and Smad7 in the stromal cells. Results The cell proliferation showed culture time dependence in all groups. The proliferation in the drug-serum treated groups was lower than that in the control group,and it was lower in the high dose treated group than in the low dose treated group (all P<0.01). The unfavorable growth did not occur morphologically after being cultured for 48 h and showed insignificant difference between various groups. Cell apoptosis was not found excepting for a few appeared in the high dose treated group (with a little amount of apoptotic cells occurring). After treatment,the expressions of TGF-β1 and Smad7 were lower in the low dose treated group and high dose treated group than in the control group (P<0.01). There was not statistical difference between the low dose treated group and high dose treated group (P>0.05). Conclusion LBXC could reduce the expressions of TGF-β1 and smad7 mRNA in stromal cells and inhibit the stromal cell proliferation,but its effect on promoting cell apoptosis is unobvious.

关闭