大黄致结肠黑变病豚鼠肿瘤坏死因子-α水平变化及意义

目的探讨大黄致结肠黑变病豚鼠血清及组织肿瘤坏死因子-α(TNF-α)水平的变化及意义。方法清洁级豚鼠112只,完全随机分为4组。正常组16只,大黄低、中、高剂量组各32只。除正常组外,大黄低、中、高剂量组分别予大黄提取液以3 g/(kg·d)、6 g/(kg·d)、12 g/(kg·d)灌胃,连续给药60天后处死各组豚鼠。应用RT-PCR法以及ELISA法,定性定量检测TNF-α水平变化及TNF-α mRNA表达。结果与正常组比较,大黄各剂量组血清、组织中TNF-α水平及TNF-α mRNA表达明显增高(P<0.01),大黄各剂量组之间比较TNF-α水平变化差异无统计学意义(P>0.05)。结论TNF-α水平随大黄致豚鼠结肠黑变病加重而呈增高趋势。

英文摘要:

Objective To study the induced change of tumor necrosis factor α(TNF-α) level in serum and colon tissue of guinea pig model with melanosis coli and its significance induced by rhubarb(RB).MethodsOne hundred and twelve guinea pigs of clean grade were randomly divided into four groups: the 16 in the normal group(untreated) and the 3 RB groups(32 in each) treated with low(3 g/kg·d),medium(6 g/kg·d),and high(12 g/kg·d) dose of rhubarb respectively,administered by gastrogavage for 60 successive days.All guinea pigs were sacrificed at the terminal of the experiment and their blood serum and colon tissue were taken for detecting TNF-α level and TNF-α mRNA expression qualitatively and quantitatively using ELISA and RT-PCR.ResultsCompared with the normal group,serum and colonic tissue levels of TNF-α and TNF-α mRNA expression in the RB groups were higher significantly(P<0.01),while no significant difference was found among the later three groups(P>0.05).ConclusionRB could induce change of TNF-α level in serum and colon tissue of guinea pig with melanosis coli.

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