消炎利胆片提取物对小鼠应激性肝损伤的影响

作者	单位
尹小萍	暨南大学中药及天然药物研究所
栗原博	暨南大学中药及天然药物研究所
宝丽	沈阳药科大学中药学院
姚新生	暨南大学中药及天然药物研究所沈阳药科大学中药学院

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中文摘要:

目的研究消炎利胆片提取物(XLP)对拘束负荷诱发小鼠应激性肝损伤的影响。方法实验动物随机分为正常对照组,拘束应激模型组,硫普罗宁组(80 mg/kg)、XLP低、中、高剂量(XLP 125、250、500 mg/kg)组,共6组,每组10只小鼠。分别用赖氏法测定小鼠血浆丙氨酸氨基转移酶(ALT)活性、硫代巴比妥酸法测定肝组织丙二醛(MDA)含量、Griess化学法测定一氧化氮(NO)含量,荧光酶标仪测定肝组织抗氧化能力指数(ORAC)、HPLC法测定谷胱甘肽(GSH)含量,比色法测定谷胱甘肽过氧化物酶(GPX-Px)、谷胱甘肽硫转移酶(GST)活性,紫外分光光度法测定肝线粒体呼吸链复合酶活性、氧化还原差光谱测定细胞色素(Cyt)a、b、c和c1含量。结果与模型组比较,XLP可以明显降低拘束负荷小鼠血浆ALT活性、肝组织MDA和NO含量,有效提高肝组织的ORAC指数、GSH含量、GPX-Px和GST活性;同时XLP可以显著增加拘束小鼠肝线粒体呼吸链复合酶Ⅱ活性,明显降低肝细胞色素含量。结论消炎利胆片提取物对拘束负荷诱发小鼠应激性肝损伤有一定的减轻作用,其作用可能与缓解拘束负荷小鼠的氧化应激状态相关。

英文摘要:

Objective To investigate the protective effects of Xiaoyan Lidan Pian extract(XLP) on restraint stress induced liver injury in mice.MethodsLiver injury mouse model was established by restraint stress.Sixty mice were equally divided into 6 groups,the normal control group,the model group,the Thiopronin group,and the three XLP groups treated with low(125 mg/kg),moderate(250 mg/kg) and high dose(500 mg/kg) XLP respectively.Effect of various treatments was evaluated by assessing alanine aminotransferase(ALT) in plasma;malondialdehyde(MDA) content in liver by thiobarbituric acid method;content of nitric oxide(NO) by Griess chemical method;hepatic antioxidant capacity index(ORAC) by fluorescent enzyme immunoassay;glutathione(GSH) content by HPLC;activity of glutathione peroxidase(GPX-Px) and glutathione S-transferase(GST) by colorimetry;activity of hepatic mitochondrial respiratory chain complex enzyme(MRCCE) by ultraviolet spectrophotometry;and contents of cytochrome a,b,c,and c1 by the redox differential spectra.ResultsAs compared with the model group,in the XLP groups,level of plasma ALT activity,liver content of MDA and NO,and contents of cytochromes were lower,while levels of ORAC index,GSH,GPX-Px and GST in liver,and MRCCE activity were higher.ConclusionXLP has definite protective effects on stressive liver injury in mice,which may be related to its action in alleviating the oxidative stress condition in mice.

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