| 肖义军,陈元仲,陈炳华,吴勇,陈建华,林振兴.红花桑寄生总黄酮提取物选择性抑制人源肿瘤细胞增殖和诱导凋亡[J].中国中西医结合杂志,2009,(2):148-152 |
| 红花桑寄生总黄酮提取物选择性抑制人源肿瘤细胞增殖和诱导凋亡 |
| Selective Effect of Nispex in Inhibiting Human Cancer Cell Proliferation and Inducing Cell Apoptosis |
| 免费下载全文 查看/发表评论 下载PDF阅读器 |
| |
| DOI： |
| 中文关键词: 红花桑寄生 黄酮 肿瘤 细胞增殖 细胞凋亡 |
| 英文关键词:Scurrula parasitica L flavonoids tumor cell proliferation cell apoptosis |
| 基金项目:福建省教育厅资助项目(No.JA05201) |
|
| 摘要点击次数: 1347 |
| 全文下载次数: 1 |
| 中文摘要: |
| 目的观察红花桑寄生总黄酮提取物(Nispex)的体外抗肿瘤效果。方法应用MTT法研究Nispex对人及小鼠多种肿瘤细胞和非肿瘤细胞增殖的抑制效果,采用细胞集落培养法研究Nispex对人源肿瘤细胞中增殖细胞群的影响;采用AO/EB荧光染色、末端原位标记(TUNEL)法以及Annexin V流式细胞术检测细胞凋亡。结果Nispex能显著抑制人源肿瘤细胞增殖和诱导人源肿瘤细胞凋亡,对肿瘤细胞中的增殖细胞群作用更为敏感。但Nispex对人源非肿瘤细胞的抑制作用不明显,对鼠源细胞在检测浓度范围内未见有作用。结论Nispex是一种对人源肿瘤细胞具有良好选择性的天然植物提取物。 |
| 英文摘要: |
| Objective To observe the in vitro anticancer effect of Nispex,the total flavonoids extract from Scurrula parasitic L.MethodsThe cell proliferation inhibitory effects of Nispex on various kinds of tumor cells or non-tumor cells in human and rats were detected with MTT assay and colony forming assay respectively,the cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining,TUNEL assay and AnnexinV-FITC/PI flow cytometry.ResultsNispex could significantly inhibit human cancer cell proliferation and induce human cancer cell apoptosis,especially to the proliferative cell group,but its inhibition on human non-tumor cell was insignificant,and showed no effect on murine cancer cells in the tested scope.ConclusionNispex is a nature plant extract which shows good selectivity for killing human cancer cell. |
| 关闭 |
扫描微信二维码，获取更多信息