糖通方对体外培养糖尿病大鼠髂动脉血管平滑肌细胞增殖的影响

作者	单位
范冠杰	广东省中医院
罗广波	广州中医药大学
唐咸玉	广东省中医院
魏华	广东省中医院
赵玲	广东省中医院
刘振杰	广东省中医院

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中文摘要:

目的用血清药理学与分子生物学相结合的方法,探讨糖通方对糖尿病大鼠模型血管平滑肌细胞增殖的抑制作用。方法选择纯种新西兰家兔,每天灌服不同剂量(18、13、8mL/kg)的糖通方中药制剂,制备含有不同药物浓度的兔血清;用组织块种植法体外培养糖尿病大鼠模型的髂动脉平滑肌细胞(VSMC)并用不同浓度含药血清进行干预;用同位素液闪测定氚标记胸腺嘧啶脱氧核糖核苷(3H-TDR)掺入量,检测含药血清对平滑肌细胞增殖的影响;流式细胞仪测定细胞周期。结果各糖通方含药血清组呈浓度依赖性地表现为3H-TDR掺入量显著减少,3个含药血清组3H-TDR掺入量均明显低于正常血清对照组(P<0.01);流式细胞仪测定各含药血清组处于S和G2/M期细胞百分比显著低于正常血清对照组(P<0.01)。结论糖通方含药血清抑制体外培养糖尿病大鼠VSMC增殖,且抑制效应呈浓度依赖性。

英文摘要:

Objective To explore the inhibition of Tangtong Recipe(TTR) on proliferation of vascular smooth muscle cells(VSMCs) in rat model of diabetes mellitus using seropharmacological and modern molecular biological technologies.Methods The drug-serum of TTR was prepared by feeding pure-breed New Zealand rabbits at various doses(18 mL/kg,13 mL/kg,and 8 mL/kg) of TTR,and used to intervene the VSMCs get from the iliac artery of diabetic model rats cultured by tissue block plantation.The impact of drug-serum on VSMCs proliferation was estimated through measuring tritiated thymine deoxy-ribonucleotide(3H-TDR) incorporation using liquid-scintillation detector,and the cell cycle was detected using flow cytometry.ResultsThe 3H-TDR incorporation in cells after intervention with various doses of TTR drug-serum were reduced significantly,in a concentration-dependent manner,to the level lower than that in the normal serum control group(P<0.01).And the percentage of S and G2/M phase cells in the drug-serum treated groups also were markedly lower than that in the normal control group(P<0.01).Conclusion Drug-serum of TTR could inhibit the proliferation of VSMC of diabetic rat in vitro in a concentration-dependent manner.

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