四种中药含药血清对大鼠离体结肠平滑肌细胞作用的筛选研究

作者	单位
石艳婷	山东大学第二附属医院
司春峰	山东大学第二附属医院
刘斌	山东大学第二附属医院
刘传勇	山东大学医学院生理学教研室
郭建强	山东大学第二附属医院

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中文摘要:

目的观察槟榔、蒲公英、莱菔子、生白术中药血清对大鼠离体结肠平滑肌细胞运动的影响。方法52只大鼠分为生理盐水组、4味中药(槟榔、蒲公英、莱菔子、生白术)低浓度、中浓度、高浓度各3组,共13组,以相应药物灌胃3天,制备含药血清。参考改进的Bitar方法制备大鼠游离的结肠平滑肌细胞,观察各中药血清及阳性对照(氯化乙酰胆碱,ACh)组引起的结肠平滑肌细胞收缩效应。结果不同浓度槟榔、蒲公英、莱菔子、生白术中药血清对结肠平滑肌细胞均存在明显的收缩效应,且各味中药血清高浓度组作用最强(P<0.05),但各作用最强组之间比较,差异无统计学意义(P>0.05)。结论不同浓度槟榔、蒲公英、莱菔子、生白术中药血清均可引起结肠平滑肌细胞有效收缩。

英文摘要:

Objective To study the kinetogenic effects of serum containing Semen Arecae,Dandelion,Semen raphani and Atractylodes macrocephala on the colonic smooth muscle cells of rats.Methods Serum containing Chinese materia medicas was made according to standard methods.Smooth muscle cells were isolated from the muscle layers of Wistar rat′s colon,referred to modified Bitar′s method.The contractile response of colonic smooth muscle cells to serum containing Chinese materia medicas(10%,50%,100% concentration) and other medicines(blank and 1×10-3mol/L acetylcholine) were separately observed.The contractility was presented by the decrease of the cell length between the drug groups and the control.Results Serum containing each Chinese materia medica can make dose-dependent contraction at different concentrations(P<0.05),but the strongest effect of each serum had no significant difference(P>0.05).Conclusion Serum containing Semen Arecae,Dandelion,Semen raphani and Atractylodes macrocephala can make notable contraction on colonic smooth muscle cells in rats.

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