冠心病气虚血瘀证的蛋白质组学特征研究

目的应用高解析离子淌度质谱与纳升级超高效液相色谱联用新技术(LC-MSE),寻找冠心病不稳定性心绞痛气虚血瘀证的血浆特征分子,探索冠心病不稳定性心绞痛气虚血瘀证在蛋白质组学层面的特征。方法采用美国Agilent公司多克隆抗体亲和柱去除冠心病不稳定性心绞痛气虚血瘀证、非气虚血瘀证患者和健康人血浆中6种丰度最高的蛋白后,进行LC-MSE分析。结果初步发现肌动蛋白(Actin)仅在冠心病不稳定性心绞痛气虚血瘀证患者血浆中表达;纤维连接蛋白(FN)、载脂蛋白H(ApoH)、膜联蛋白(ANXA6)仅在冠心病不稳定性心绞痛气虚血瘀证患者中高表达。此外,与健康人比较,血清淀粉样蛋白(SAA)、铜蓝蛋白(CP)、肌球蛋白H11(MYH11)及补体C6在冠心病不稳定性心绞痛气虚血瘀证患者中高表达;载脂蛋白A4(ApoA4)、凝溶胶蛋白(GSM)、血红蛋白B(HBB)及转铁蛋白(TF)等6种蛋白在冠心病不稳定性心绞痛气虚血瘀证患者中低表达。结论冠心病不稳定性心绞痛气虚血瘀证可能属于一种炎症反应;冠心病不稳定性心绞痛气虚血瘀证患者可能同时存在心肌损伤、凝血因子异常、脂代谢紊乱与氧运输障碍;这些方面相互影响,互为因果。新发现的差异蛋白可能为研究或发现抗心绞痛药物作用的新靶标提供线索。这种非标记定量蛋白质组学新技术是疾病及证候生物标志物研究的有效手段。

英文摘要:

Objective To seek the special plasma molecule in unstable angina (UA) patients of qi deficiency and blood stasis syndrome (QDBS) and method for explore the proteomic specificity of the disease. Methods LC-MSE analysis was performed in UA patients of QDBS or non-QDBS and in healthy persons after the 6 proteins with optimal abundance in plasma being removed by polyclonal antibody affinity column (product of Agilent Co. USA). Results Actin were found only expressed,and FN,ApoH and ANXA6 were found highly expressed in plasma of patients with UA-QDBS,suggesting they might be the special molecules for the disease. Moreover,as compared with health persons,SAA,CP,MYH11 and C6 showed high expression,and the 6 proteins,e.g. A1BG,ApoA4,GSN,HBB,HBD and TF,showed low expression in the plasma of UA-QDBS patients. Conclusion UA-QDBS might belong to a kind inflammatory reaction. There are simultaneous existence of myocardial injury,blood coagulation factor abnormality,lipid metabolic disorder and oxygen transport obstacle in patients of UA-QDBS,they influence and interact mutually. The newly discovered differential proteins might provide clues for studying or discovering new protein targets of angina relieving drugs. The new technique of label free quantitative proteomics is an efficient method for bio-marker research of diseases and syndromes.

关闭