| 李莉,王俊锋,熊大经.固肾培元方对肾阳虚大鼠耳蜗组织Bcl-2及Bax mRNA表达的影响[J].中国中西医结合杂志,2009,(6):533-536 |
| 固肾培元方对肾阳虚大鼠耳蜗组织Bcl-2及Bax mRNA表达的影响 |
| Effect of Gushen Peiyuan Recipe on Bcl-2 and Bax mRNA Expressions in Cochlea Tissue of Rats with Shen-Yang Deficiency Syndrome |
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| DOI： |
| 中文关键词: 固肾培元方 肾阳虚 耳蜗 Bcl-2 mRNA Bax mRNA 肾与耳 |
| 英文关键词:Gushen Peiyuan Recipe Shen-yang-deficiency cochlea Bcl-2 mRNA Bax mRNA kidney and ear |
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| 中文摘要: |
| 目的观察固肾培元方对肾阳虚大鼠耳蜗组织Bcl-2、Bax mRNA蛋白表达的影响。方法采用肌肉注射氢化可的松注射液的方法造成SD大鼠肾阳虚模型;空白组及模型组10mL/kg生理盐水灌胃,中药高、中、低剂量组分别给予固肾培元方31g/kg、15.5g/kg及7.75g/kg灌胃;用RT-PCR的方法检测耳蜗组织Bcl-2、Bax mRNA表达。结果固肾培元方可使肾阳虚大鼠耳蜗Bcl-2 mRNA表达增强,Bax mRNA表达减弱,Bcl-2/Bax比例增加,与空白组比较,差异无统计学意义(P>0.05)。结论固肾培元方可通过调节耳蜗相关凋亡因子Bcl-2、Bax mRNA的表达而达到改善听力、保护耳蜗结构与功能的目的。 |
| 英文摘要: |
| Objective To study the effect of Gushen Peiyuan Recipe (GPR) on the expression of Bcl-2 and Bax mRNA in Shen-yang-deficiency (SYD) rats suffering from cochlea apoptosis,thus providing a theoretical basis to the treatment and prevention of sensorineural hearing loss and fill Chinese medine′s theory of kidney-ear-correlation with new substance. Methods Rats were induced into experimental SYD animal models by injecting cetacort into their buttocks. Rats in the blank and model groups were given 10 mL/kg normal saline by gastrogavage,and 31 g/kg,15.5 g/kg and 7.5 g/kg GPR were given to the rats in the high,medium and low dose groups by gastrogavage respectively. RT-PCR was adopted to detect the mRNA expressions of Bcl-2 and Bax. Results Levels of Bcl-2 mRNA expression and Bcl-2/Bax enhanced,and mRNA expression of Bax attenuated in the model rats after GPR treatment,the Bcl-2/Bax ratio increased,showing insignificant difference when compared with the blank control group (P>0.05). Conclusion GPR plays a significant role in regulating the mRNA expressions of Bcl-2 and Bax,therefore improving the hearing of SYD rats and protecting the structure and function of cochlea. |
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