天癸更年软胶囊对雌性老年大鼠脑保护作用及机制

作者	单位
李媛洁	西安交通大学天奎医药生物公司西安交通大学医学院人体解剖与组织胚胎学系
宋天保	西安交通大学医学院人体解剖与组织胚胎学系
赵璇	西安交通大学医学院人体解剖与组织胚胎学系
蔡艳艳	西安交通大学医学院人体解剖与组织胚胎学系
宋鑫	西安交通大学医学院药学系
陈东	西安交通大学天奎医药生物公司
宋兰兰	西安交通大学天奎医药生物公司
魏霞蓁	西安交通大学天奎医药生物公司

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中文摘要:

目的探讨天癸更年软胶囊(含沙棘脂肪酸)的脑保护和抗衰老作用及其机制。方法选用老年(16月龄)雌性大鼠,给予低、中、高剂量(0.72、1.80、4.5g/kg)天癸更年软胶囊灌胃180天,同时设立雌激素、老年和青年(3月龄)对照组。应用免疫组织化学技术对各组大鼠脑组织的雌激素β受体(ERβ)、酪氨酸羟化酶(TH)、粒细胞集落刺激因子(G-CSF)和血管内皮生长因子(VEGF)的表达进行观察和分析。应用尼氏染色监测神经元和胶质细胞的数量变化。结果免疫组织化学研究显示,天癸更年软胶囊可显著增加雌性老年大鼠下丘脑ERβ阳性和TH阳性神经元数(P<0.01,P<0.05),使脑内星形胶质细胞的G-CSF染色明显减弱(P<0.01),并显著增强脑血管的VEGF表达(P<0.05)。尼氏染色显示各组间神经元和胶质细胞数差异无统计学意义。结论天癸更年软胶囊可增加雌性老年大鼠脑组织ERβ、TH和VEGF的表达,调节星形胶质细胞反应及其G-CSF表达,这可能与其神经保护和抗衰老作用有关。

英文摘要:

Objective To investigate the brain-protective and anti-aging effects and the mechanism of action of Tiangui Gengnian Soft Capsule(TGSC),a Chinese herbal preparation composed of seabuckthorn fatty acids.Methods Sixteen-month-old female rats were administered via gastric perfusion with low,medium and high doses(0.72 g/kg,1.80 g/kg and 4.5 g/kg)of TGSC for 180 days.The 3-month and 22-month old rats were taken as controls.Expression of estrogen receptor β(ERβ),tyrosine hydroxylase(TH),granulocyte colony-stimulating factor(G-CSF)and vascular endothelial growth factor(VEGF)in the brain of rats were observed and analyzed using immunohistochemical technique,and changes in the number of neurons and glial cells were counted with Nissl’s staining.Results Immunohistochemistry showed the number of ERβ-and TH-positive neurons in the hypothalamus was significantly higher(P<0.01 and P<0.05),G-CSF-positive astrocyte stain was much weaker(P<0.01)and the expression of VEGF-positive cerebral vessel was stronger(P<0.05)in the TGSC treated groups than those in the control.Nissl’s staining showed no significant difference in the number of neurons and glial cells among groups.Conclusion TGSC could up-regulate the expressions of ERβ,TH and VEGF in the brain of aged female rats,modulate the responsibility of astrocyte and its G-CSF expression,suggesting that TGSC may have certain neuro-protective and anti-aging effects.

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