补肾助孕方对大鼠胚胎着床期子宫内膜ER、PR及整合素α<sub>5</sub>、β<sub>3</sub>蛋白表达的影响

Effects of Bushen Zhuyun Recipe on Protein Expressions of Estrogen Receptor,Progesterone Receptor and Integrin α₅ and β₃ in Endometrium of Rats at the Implantation Stage

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中文关键词: 补肾助孕方胚胎着床期雌激素受体孕激素受体整合素α5 整合素β3 子宫内膜容受性受孕率

英文关键词:Bushen Zhuyun Recipe embryo implantation stage estrogen receptor progesterone receptor integrin α5 integirn β3 endometrial receptivity pregnant rate

基金项目:江苏省自然科学基金资助项目(No:BK2006240)

作者	单位
周惠芳	南京中医药大学
李爱萍	南京医科大学
谈勇	南京中医药大学

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中文摘要:

目的观察补肾助孕方对大鼠胚胎着床期子宫内膜细胞雌激素受体(ER)、孕激素受体(PR)及整合素α5、β3蛋白表达的影响,以阐明该方改善黄体功能不全性不孕症的机制。方法将SD雌性大鼠随机分成空白组、模型组、西药组和中药补肾助孕方低、中、高剂量组,分别对各组大鼠由妊娠至胚胎着床期处死,取子宫中段组织(约1cm),采用Western blot技术分析各组大鼠胚胎着床期子宫内膜ER、PR及整合素α5、β3的蛋白表达等。结果模型组子宫内膜ER、PR的蛋白表达明显高于空白组,补肾助孕方低、中剂量组明显低于模型组(P<0.05);模型组子宫内膜整合素α5、β3的蛋白表达明显低于空白组,补肾助孕方中、高剂量组明显高于模型组(P<0.05)。结论补肾助孕方可下调胚胎着床期子宫内膜ER、PR的水平,提高整合素α5、β3水平的表达,改善黄体功能不全状态子宫内膜的容受性,从而创造了良好的受孕环境。

英文摘要:

Objective To observe the effects of Bushen Zhuyun Recipe(BZR)on protein expressions of estrogen receptor(ER),progesterone receptor(PR)and integrin α5 and β3 in endometrium of rats at the implantation stage,for exploring the possible mechanism of the recipe in treating luteal phase defect(LPD)infertility.Methods Female SD rats were randomly divided into 6 groups,the blank group,the model group,the WM group treated by Western medicine,and the three BZR groups treated by low-,middle-and high-dose BZR respectively.Rats were made to pregnancy and sacrificed at the implantation stage,their middle segment of uterus,about 1 cm in length was gotten for detecting the protein expressions by Western blot.Results The protein expressins of endometrial ER and PR were significantly higher,while those of integrin α5 and β3 were significantly lower than those of the control group(P<0.05).The protein expressions of endometrial ER and PR were significantly lower,but those of integrin α5 and integrin β3 were higher in rats treated by middle-and high-dose BZR than those in model rats(P<0.05).Conclusion BZR can raise the receptivity of rats’ endometrium through down-regulating the expressions of ER,PR and increasing the protein expression of integrin α5 and β3 in endometrium and thus to enhance the pregnant rate.

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