葛根素抑制卵巢癌细胞HO-8910侵袭及转移的实验研究

作者	单位
韩洁	上海长海医院中医科
俞超芹	上海长海医院中医科
沈慰	上海长海医院中医科
王针织	上海长海医院中医科

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中文摘要:

目的观察葛根素对卵巢癌HO-8910细胞黏附、侵袭人工重组基底膜(matrigel)、趋化性运动能力的影响及可能的作用途径。方法采用聚合酶链式反应(polymerase chain reaction,PCR)方法检测HO-8910细胞内雌激素受体(estrogen receptor,ER)表达的情况;四甲基偶氮唑盐比色法(MTT法)检测葛根素对卵巢癌细胞HO-8910细胞增殖的影响;细胞黏附人工重组基底膜实验检测葛根素对HO-8910细胞黏附能力的影响;聚碳酸酯膜细胞培养小室(Transwell小室)法检测葛根素对HO-8910细胞的侵袭能力和趋化运动能力。结果HO-8910细胞阳性;细胞黏附实验中,20μmol/L葛根素处理细胞12h后OD值显著低于溶剂对照组(P<0.01),抑制率达50.63%;Transwell小室侵袭、趋化性运动实验中,20μmol/L葛根素处理细胞12h后穿膜细胞数均明显低于溶剂对照组(P<0.01),抑制率分别达到38.59%、40.63%;葛根素与雌激素联合干预组的穿膜细胞数比雌激素组明显减少[分别为(33.40±3.30)vs(48.05±3.56)(P<0.01);(35.35±3.03)vs(52.45±1.04)(P<0.01)]。结论葛根素可抑制人卵巢癌细胞株HO-8910黏附、侵袭和运动的能力,可拮抗雌激素对细胞恶性行为的刺激作用。

英文摘要:

Objective To investigate the inhibitory effect of puerarin on invasive and metastatic abilities of tumor cells,and its possible mechanism through observing its impacts on the migratory,adhesive and invasive capacities of human oophoroma cells HO-8910 to the artificial recombined basement membrane.Methods Expression of estrogen receptor(ER)in HO-8910 cells was detected using PCR assay.Effects of puerarin on HO-8910 proliferation was detected with MTT assay;on its adhesion potential was tested with cell-matrigel adhesion assay,and on invasive and migratory capacities were measured with Transwell matrigel invasion assay and Transwell motility assay respectively.Results ER was positively expressed in HO-8910 cells.After being treated with 20 μmol/L puerarin for 12 h,the adhesive test showed that OD value in the tested group was significantly lower than that in the control(P<0.01),the inhibiting rate reached 50.63%;and the Transwell assay showed a significant lowering of penetrated cells(P<0.01),the inhibition rate for invasion was 38.59% and that for motility migration 40.63%.The number of penetrated cells was lower in the group intervened with combination of Puerarin and estrogen than in the group intervened with estrogen alone,33.40±3.30 vs 48.05±3.56 for invasion and 35.35±3.03 vs 52.45±1.04 for motility(all P<0.01).Conclusion Puerarin can inhibit the adhesion,invasion and migration of HO-8910 cells,plays an antagonist effect against the stimulation of estrogen on the malignant behavior of tumor cells.

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