广藿香对肢体缺血-再灌注大鼠肠上皮细胞膜流动性的保护作用

目的观察广藿香对肢体缺血-再灌注大鼠血清一氧化氮(NO),肿瘤坏死因子(TNF-α)及肠上皮细胞膜流动性的影响,探讨广藿香保护肠屏障功能的作用及机制。为从中医药角度寻找创伤、手术后等胃肠道保护措施新方法提供实验依据。方法成年Wistar大鼠80只,建立肢体缺血-再灌注模型。将实验动物随机分为正常对照组,模型组,广藿香水提液高(4g·kg-1.d-1)、中(3g·kg-1.d-1)、低(2g·kg-1.d-1)剂量组以及广藿香挥发油高(4g.kg-1.d-1)、中(3g.kg-1.d-1)、低(2g·kg-1.d-1)剂量组。各组在造模前连续5天灌服相应药物。检测血清NO及TNF-α浓度变化;DPH荧光探针技术测定肢体缺血再灌注后肠上皮细胞膜流动性的变化。结果与正常组比较,模型组大鼠血清NO浓度和TNF-α水平显著升高(P<0.01),肠上皮细胞细胞膜荧光偏振度P值升高(P<0.05)。与模型组比较,给药各组大鼠血清NO浓度及TNF-α水平显著降低(P<0.01或P<0.05);藿香水提液低剂量组及藿香挥发油中剂量组肠上皮细胞细胞膜荧光偏振度P值降低,与模型组比较差异有统计学意义(P<0.05)。结论广藿香可以通过降低血清NO浓度,抑制血清TNF-α水平,使肠上皮细胞保持良好的细胞膜流动性,达到保护肠屏障功能的作用。

英文摘要:

Objective To observe the effects of Pogostemon cablin(Blanco)Benth.(PCB),a Chinese aromatic herbal medicine,on serum levels of nitric oxide(NO),tumor necrosis factor-α(TNF-α),and membrane fluidity of intestinal epithelial cells(IMC)in rats undergoing lower limbs ischemic reperfusion(I/R),for exploring its action in protecting intestinal barrier and the possible mechanisms,and to seek a new way,viewing from Chinese medicine,for providing the experimental bases of gastrointestinal protection against trauma or surgical operation.Methods Eighty adult Wistar rats were induced into lower limb I/R model and randomized equally into the model group,the three PCB water extract groups treated respectively with high-(4 g·kg-1·d-1),middle-(3 g·kg-1·d-1),and low-dose(2 g·kg-1·d-1)of PCB water extract,and three PCB volatile oil groups treated respectively with high-(4 g·kg-1·d-1),middle-(3 g·kg-1·d-1),low-dose(2 g·kg-1·d-1)of PCB volatile oil.Besides,10 healthy rats was allocated in a normal control group.PCB preparation was given via gastric infusion for 5 successive days just before modeling.The serum levels of NO and TNF-α were monitored,and the membranous fluidity of IMC at I/R region was determined by fluorescence polarization technique.Results Compared with the control group,both serum NO and TNF-α levels in model rats were significantly higher(P<0.01),and the fluorescence polarization value(P)of IMC obviously increased at the same time(P<0.05).As compared with the model group,the serum level of NO and TNF-α significantly reduced in all the PCB treated groups(P<0.01 or P<0.05).As for the membrane fluidity,significant difference was shown between the model group with low-dose of PCB water extract and middle-dose of PCB volatile oil(P<0.05).Conclusion PCB could effectively protect the intestinal barrier function by way of maintaining the membrane fluidity of IMC through regulating the level of NO and TNF-α in serum.

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